Affiliates severe sepsis were consistent Ver Changes in the infected fish, which may need during the experimental period were killed observed. Mononuclear Re Inflammatory infiltrates were primarily in the brain, heart, and the retrobulb Localized upper area of the eye. Multifocal necrosis in the brain and the heart was examined fish, especially in convalescence and chronically infected. There was gsk3b inhibitor no association between the H Frequency and intensity t of histopathological changes Ver And tested different modes of infection. No microscopic Ver Changes were observed in fish from the control group. 4th In the discussion period M March 2008 to M March 2009, three F ll Of h Hemorrhagic septic Chemistry by Weissella sp. were in the rainbow trout on commercial farms located in different Brazilian states found.
This is the first report of several F Ll of Weissella sp. Infection in farmed fish and au Adopted outside of China. W During epidemics was the clinical picture of the disease Similar to the previously described rainbow trout in Asia. However, were h Mortality here Tsraten observed in our study. The main pr Cyclopamine Hedgehog inhibitor Predisposing factor associated with the disease was the Erh Increase in water temperature. Although observed in the first adult fish under field conditions Weissella sp. k can also cause disease in rainbow trout fry. Therefore, the disease is a potential problem for hatcheries and commercial farms. The Brazilian isolates showed a ph Phenotypic profile was consistent, but some differences compared to biochemical profiles of the six Chinese Weissella sp observed. St Strains.
Brazilian St Mme pr presents An increase of 45 8C and has variable hydrolysis of arginine and esculin hydrolysis positive, in contrast to the fish isolates previously isolated and characterized. Traditionally, the identification of Rapamycin lactic Acid bacteria using ph Phenotypic criteria. However, this standard methodology does not allow clear discrimination of the new St Strains of Weissella. To avoid confusion, the isolates were subjected to molecular analysis, they showed strong genetic Similarity with Chinese St Strains, which means that all St Mme k Nnten the same bacterial species to be. In addition, the Brazilian Weissella sp. Isolates were grouped in a phylogenetic lineage that is closely related to W. halotolerans.
However, based on PH Are phenotypic and molecular results, there are insufficient data to classify them as the same bacterial species. Further studies are needed to Brazilian clades Characterize the species level. The genetic diversity of isolates from different farms is unknown and should be addressed. Bearer of the hunter-diffusion test remains the determination of sensitivity to antibiotics most used in clinical microbiology. However, some problems with the interpretation of reported data, particularly in pathogens of aquatic animals. Recently, a method called normalized resistance interpretation was developed and used to determine epidemiological thresholds for pathogenic bacteria of the fish. A previous study reported high levels of resistance to antibiotics of Weissella sp. St mme Isolated from diseased rainbow trout. However, the methods and criteria for resistance and susceptibility to determine t of isolates is not clear. In our breeding