Major fatty acids of strain CC-SAMT-1T are summarized in the species description. As evidenced by the 16S rRNA gene sequence analysis, strain CC-SAMT-1T belonged to the family Flavobacteriaceae, phylum Bacteroidetes, and formed discrete phyletic line distantly associated with Mariniflexile species (Fig. 2). Strain CC-SAMT-1T was clearly distinguished from Mariniflexile species principally based on its additional unidentified aminolipid (AL2–4) and glycolipid (GL) contents (Fig. 3, Figs S2 and S3). Furthermore, strain CC-SAMT-1T can also be differentiated selleck chemicals llc from phylogenetic neighbors by fatty acid profiles (Table 2 and Table S2) and several phenotypic
features (Table 1 and Table S1). Thus, based on the polyphasic data, strain CC-SAMT-1T represents a novel genus and species of the family Flavobacteriaceae, for which the name Siansivirga zeaxanthinifaciens gen. nov., sp. nov. is proposed. Si.an.si.vir’ ga. N.L. n. Siansi, a township in Taiwan, L. fem. n. virga stick, N. L. fem. n. Siansivirga stick of Siansi. buy Galunisertib Cells are Gram-negative, strictly aerobic, nonspore-forming, chemoheterotrophic, and mesophilic; catalase- and oxidase-positive. Cells are typically rod-shaped with rounded ends, nonflagellated, and motile by gliding. Zeaxanthin is the predominant xanthophyll. Flexirubin-type pigments
are absent. Major isoprenoid quinone is MK-6. The major fatty acids are iso-C15:0 (14.8%), iso-C17:0 3-OH (11.8%), iso-C15:1 G (10.6%), anteiso-C15:0 (9.7%), C16:0 (8.1%), iso-C16:0 3-OH (7.9%), iso-C15:0 3-OH (7.5%), and summed feature 3 containing C16:1 ω6c and/or C16:1 ω7c (7.5%). PE, four unidentified aminolipids four unidentified lipids, and an unidentified glycolipid are the polar lipids. The DNA G+C content of the type strain of the type species is 33.7 mol%. As determined by 16S rRNA gene sequence analysis, the genus Siansivirga is a novel member of the family Flavobacteriaceae.
The type species is S. zeaxanthinifaciens. Siansivirga zeaxanthinifaciens (ze.a.xan.thi.ni.fa’ci.ens. N.L. Anidulafungin (LY303366) neut. n. zeaxanthinum zeaxanthin; L. part. pres. faciens making/producing; N.L. part. adj. zeaxanthinifaciens zeaxanthin-producing). Cells are 0.3–0.8 μm in diameter and 0.6–6.2 μm in length. On MA, after 1–2 days of incubation at 30 °C, it forms small, circular, convex, and intense yellow-colored colonies (0.5–1.0 mm in diameter). Colony color may turn orange after prolonged incubation because of intense cellular accumulation of zeaxanthin. Growth is observed between 15 and 37 °C (optimum, 30 °C), pH 5.5–8.5 (optimum, 7.0–8.0), and 1–4% NaCl (optimum, 2–3%). Chitin, starch, Tween 20 and Tween 80 are hydrolyzed, whereas casein, CMC, xylan, DNA, and l-tyrosine are not.