On the basis of the microscopic and immunohistochemical findings, we made diagnosis of diffuse large B cell lymphoma.

15 days after primary closure, patient underwent subtotal gastrectomy and then chemotherapy was performed. Patient is during the follow up without any particular complication. Key Word(s): 1. Gastric lymphoma; 2. perforation Presenting Author: Birinapant chemical structure SHOU WU LEE Additional Authors: HAN CHUNG LIEN, CHI CHEN LIN, MEI SIN PAN, MING HSIEN LIN, KAREEN CHONG, CHI SEN CHANG, CHUNG HSIN CHANG Corresponding Author: SHOU-WU LEE Affiliations: Taichung Veterans General Hospital, National Chung Hsing University, Taichung Veterans General Hospital, Taichung Veterans General Hospital, Taichung Veterans General Hospital, Taichung Veterans General Hospital, Taichung Veterans General Hospital Objective: Transforming growth factor β (TGF- β) overexpression or signaling dysfunction has been demonstrated for many tumors. The aim of this study was to investigate the expression of TGF- β in the epithelium of human Barrett’s esophagus (BE). Methods: Patients with endoscopic

suspected BE (ESBE) were collected between November 2012 and March 2013. Biopsy over BE epithelium was applied and classified by an histopathologist. Reverse transcription-polymerase chain reaction (RT-PCR) for TGF- β mRNA expression over biopsy IWR-1 order specimen from normal squamous epithelium of esophagus, BE, and gastric cardia were assessed. Four human esophageal cell lines, including HETA1 (normal), CA-A (BE without dysplasia), CP-C (BE with dysplasia) and OE-33 (Adenocarcinoma), were selected. RT-PCR for mRNA and western blotting for protein of TGF- β expression of each cell were assessed. Results: Among all thirty enrolled

patients, twenty-eight, one, and one cases were medchemexpress classified as BE without dysplasia, BE with low-grade dysplasia and BE associated AC, respectively. Expression of TGF- β mRNA in the epithelium of BE, with or without intestinal metaplasia, dysplasia, or AC, was significantly (P -value < 0.01) lower than that in normal esophagus and gastric cardia. In the cell line, expression of TGF- β mRNA and protein were significantly (P -value < 0.05) lower from BE and associated adenocarcinoma than that from normal esophagus. Conclusion: The expression of TGF- β was low in the epithelium of BE irrespective of the presence of dysplasia or associated adenocarcinoma. This finding provided a potential biomarker for diagnosis of BE. Key Word(s): 1. Barrett’s esophagus; 2. dysplasia; 3.