minimum frequency of occurrence within the 24 single spectra was set to 50% for every mass. Peak lists of MSP were exported for further evaluation. Acknowledgements We are grateful to Kerstin Cerncic, Renate Danner, Byrgit Hofmann, and Karola Zmuda for their excellent technical assistance. Electronic supplementary material Additional file 1: Table S2: Results of VNTR, SNP, INDEL analysis and erythromycin sensitivity testing of Francisella tularensis subsp. holarctica isolates. The number of repeats is given for Ft-M3a, Ft-M3b, and Ft-M6. The number of base-pairs is given for Ft-M24. Derived state of SNPs and INDELs is in boldface. Nomenclature is according to Karlsson et al. (2013) , where the B.I clade was re-defined to include both B1 and B3  (DEL, deletion; IN, insertion; bp, basepairs; BW – Baden-Württemberg, www.selleckchem.com/products/GSK1904529A.html BY – Bavaria, NRW – North Rhine-Westphalia, LS – Lower Saxony, SN – Saxony, TH – Thuringia; n.d., not done). (XLSX 16
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