0 The

minimum frequency of occurrence within the 24 sing

0. The

minimum frequency of occurrence within the 24 single spectra was set to 50% for every mass. Peak lists of MSP were exported for further evaluation. Acknowledgements We are grateful to Kerstin Cerncic, Renate Danner, Byrgit Hofmann, and Karola Zmuda for their excellent technical assistance. Electronic supplementary material Additional file 1: Table S2: Results of VNTR, SNP, INDEL analysis and erythromycin sensitivity testing of Francisella tularensis subsp. holarctica isolates. The number of repeats is given for Ft-M3a, Ft-M3b, and Ft-M6. The number of base-pairs is given for Ft-M24. Derived state of SNPs and INDELs is in boldface. Nomenclature is according to Karlsson et al. (2013) [16], where the B.I clade was re-defined to include both B1 and B3 [15] (DEL, deletion; IN, insertion; bp, basepairs; BW – Baden-Württemberg, www.selleckchem.com/products/GSK1904529A.html BY – Bavaria, NRW – North Rhine-Westphalia, LS – Lower Saxony, SN – Saxony, TH – Thuringia; n.d., not done). (XLSX 16

KB) References 1. Johansson A, Farlow J, Larsson P, Dukerich M, Chambers E, Byström M, Fox J, Chu M, Forsman M, Sjöstedt A, Keim P: Worldwide genetic relationships among Francisella tularensis isolates determined by multiple-locus variable-number tandem repeat analysis. J Bacteriol 2004, 186:5808–5818.PubMedCrossRef 2. Keim P, Johansson A, Wagner DM: Molecular epidemiology, evolution, and ecology of Francisella . Ann

N Y Acad Sci 2007, 1105:30–66.PubMedCrossRef 3. Petersen JM, Schriefer ME: Tularemia: emergence/re-emergence. Vet Res 2005, BKM120 clinical trial 36:455–467.PubMedCrossRef 4. Ellis J, Oyston PC, Green M, Titball RW: Tularemia. Clin Microbiol Rev 2002, 15:631–646.PubMedCrossRef 5. Knothe H: Epidemiology of tularemia. Beitr Hyg Epidemiol 1955, 7:1–122.PubMed 6. Grunow R, Priebe H: Tularämie – zum Vorkommen in Deutschland. Epidemiol Bull 2007, 7:51–56. 7. www.selleck.co.jp/products/E7080.html Splettstoesser WD, Mätz-Rensing K, Seibold E, Tomaso H, Al Dahouk S, Grunow R, Essbauer S, Buckendahl A, Finke EJ, Neubauer H: Re-emergence of Francisella tularensis in Germany: fatal tularaemia in a colony of semi-free-living marmosets ( I-BET151 manufacturer Callithrix jacchus ). Epidemiol Infect 2007, 135:1256–1265.PubMedCrossRef 8. Hofstetter I, Eckert J, Splettstoesser W, Hauri AM: Tularaemia outbreak in hare hunters in the Darmstadt-Dieburg district, Germany. Euro Surveill 2006., 11: E060119.3 9. Splettstoesser WD, Tomaso H, Al Dahouk S, Neubauer H, Schuff-Werner P: Diagnostic procedures in tularaemia with special focus on molecular and immunological techniques. J Vet Med B Infect Dis Vet Public Health 2005, 52:249–261.PubMedCrossRef 10. Johansson A, Berglund L, Eriksson U, Göransson I, Wollin R, Forsman M, Tärnvik A, Sjöstedt A: Comparative analysis of PCR versus culture for diagnosis of ulceroglandular tularemia. J Clin Microbiol 2000, 38:22–26.PubMed 11.

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