01, except for pKL-1, with p < 0.05, and the pKLC conserved hypothetical protein, which does

not show a statistically significant correlation) [14]. The function of most of the genes belonging to this island has not been deciphered yet, but it is known that the PAPI-1/pKLC102-like members encode virulence factors, such as cytotoxins, pili, fimbriae and regulators of biofilm synthesis and antibiotic resistance [27]. Given the known functions of this island, the identified positive correlation to chronic infections was unexpected, as it has been demonstrated that P. aeruginosa reduces its acute virulence during the adaptation to the CF lung environment [28]. Nevertheless, Rakhimova and collaborators [14] showed that the pKL-3 gene was associated to a prolonged colonization time in a minority of P. aeruginosa strains in COPD patients [14], whose lung Batimastat colonization Ganetespib manufacturer pattern by www.selleckchem.com/products/shp099-dihydrochloride.html Pseudomonas strains is comparable to the one observed in CF patients. Analysis of the AT-genotypes identified within the publicly available population studies An intrinsic feature of the AT technology is to be standardized and therefore to guarantee reliable data comparison between genotyping studies performed worldwide in different laboratories [7]. In order to gain further information on the

124-independent strains of our collection, we compared them with a global database, obtained by retrieving information from 4 publicly available AT-datasets, comprising a total of 698 isolates [7, 14, 15, 17]. These datasets comprised 240 strains of diverse Lepirudin habitat and geographic origin [7], 134 strains collected from patients affected by chronic obstructive pulmonary disease [14], 63 strains isolated from keratitis [15], and 381 environmental isolates from rivers [17]. Our 124-independent strain collection included 27 genotypes previously described [7, 14, 15, 17] and 14 which have never been previously reported (see Table 1). Among the 27 already described AT-genotypes, it is interesting to notice that 8 of them (D421, 3C2A, C40A,

2C1A, 239A, 0812, E429 and F429) were shared by all collections [7, 14, 15, 17] and were all among the 16 most abundant in the global P. aeruginosa population [7]. An eBURST analysis using 15 markers (13 SNPs, the multiallelic fliCa/fliCb locus and exoS/exoU) was performed to illustrate the similarities between SNP profiles of our and other collections, typed by the AT method. As shown in Additional file 6, the eBURST analysis revealed the presence of 2 main clusters of clones and 3 small ones (with 2–3 genotypes each). Most AT clones also previously described (25 out of 27) belonged to the 2 large clusters, 12 of which were among the 16 most abundant clones in the global P. aeruginosa population, namely D421, F469, 1BAE, 2C1A, 0C2E, 239A, 0812, C40A, E429, EC29, F429 and 3C2A [7]. All novel AT clones except one (1E1E) were part of the 2 large clusters or gave rise to a small cluster including a previously described strain (i.e.