The PNPLA3 polymorphism is also associated with susceptibility to HCC in patients with other causes of hepatitis.[34, 43] Our data suggest that the PNPLA3 rs738409 selleck screening library polymorphism may provide important information that will assist identification of patients at particular risk
for HCC. In the present study, early age at onset of HCC was also independently associated with male sex and higher BMI, and the median interval between blood transfusion and the onset of HCC was significantly associated with male sex. These results are consistent with previous reports of male sex and higher BMI as independent risk factors for HCC development in CHC patients.[9, 44, 45] A limitation of the present study is its retrospective design. The histology samples at the time of initial treatment were obtained via ultrasound-guided aspiration at the time of percutaneous tumor ablation or surgical resection. To minimize the risk of bleeding, ultrasound-guided aspiration selleckchem was not performed for patients with a platelet
count of less than 6 (×104/μL). Therefore, the histological samples were collected from a biased group of patients. Another limitation is the cross-sectional study design and the lack of controls without HCC. We are unable to confirm whether the age at onset of HCC (primary outcome of the present study) is an adequate indicator of susceptibility to HCC from the current study alone. Further prospective study is needed to validate the current results. In conclusion, the PNPLA3 rs738409 C>G polymorphism may play a significant role in hepatocarcinogenesis
in CHC patients. Thus, this genetic factor should be taken into consideration when determining a treatment strategy intended to prevent the future development of HCC in CHC patients. THIS STUDY WAS supported by the Global COE Program, “Center of Education and Research for Advanced Genome-Based Medicine: For personalized Amino acid medicine and the control of worldwide infectious diseases”; the Ministry of Education, Culture, Sports, Science and Technology, Japan; by grants from the Leading Project of the Ministry of Education, Culture, Sports, Science and Technology, Japan; and by Health and Labor Sciences Research Grants for Research on Hepatitis from the Ministry of Health, Labor and Welfare, Japan. “
“Aim: Human embryonic stem cells (hESCs) are able to self-renew and differentiate into a variety of cell types. Although miRNAs have emerged as key regulators in the cellular process, a few studies have been reported about behaviors of miRNAs during differentiation of hESCs into a specialized cell type. Here, we demonstrate that different kinds of miRNAs may function in a lineage-specific manner during the differentiation of human embryonic stem cells (hESCs). Methods: hESCs were induced to definitive endoderm (DE) cells and further differentiated to hepatocytes. The expression levels of miRNAs were examined in hESCs, DE cells, and hepatocytes by miRNA array using 799 human miRNA probes.