Agudo D, Mendoza MT, Castanares C, Nombela C, Rotger R: A proteomic approach to study Salmonella typhi periplasmic proteins altered by a lack of the DsbA thiol: Disulfide isomerase. Proteomics 2004, 4:355–363.CrossRefPubMed 41. Natale P, Bruser T, Driessen AJM: Sec- and Tat-mediated protein secretion across the bacterial cytoplasmic membrane – Distinct translocases and mechanisms. Biochim Biophys Acta 2008, 1778:1735–1756.CrossRefPubMed 42. Jeffery CJ: Moonlighting proteins. Trends Biochem Sci

1999, 24:8–11.CrossRefPubMed 43. Hult K, Berglund P: Enzyme promiscuity: mechanism and applications. Trends Biotech 2007, 25:231–238.CrossRef 44. Hoiseth SK, Stocker BAD: Aromatic-Dependent Salmonella -Typhimurium Are Non-Virulent and Effective As Live Vaccines. Nature 1981, 291:238–239.CrossRefPubMed 45. Lee CA, Falkow S: The Ability

SU5402 of Salmonella to Enter Mammalian-Cells Is Affected by Bacterial-Growth State. Proc Natl Acad Sci USA 1990, 87:4304–4308.CrossRefPubMed 46. Sambrook J, Russel DW: Molecular cloning: a laboratory manual 3 Edition Cold Spring Harbor: Cold spring Harbor Laboratory Press 2001. 47. Aldridge P, Karlinsey JE, Becker E, Chevance FFV, Hughes KT: Flk prevents premature secretion of the anti-sigma factor FlgM into the periplasm. Mol Microbiol 2006, 60:630–642.CrossRefPubMed 48. Datsenko KA, Wanner STA-9090 concentration BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 2000, 97:6640–6645.CrossRefPubMed 49. Geddes K, Cruz F, Heffron F: Analysis of cells targeted by Salmonella type III secretion in vivo. PLoS Pathog 2007, 3:2017–2028.CrossRef 50. Uzzau S, Figueroa-Bossi N, Rubino S, Bossi L: KU-57788 purchase Epitope tagging of chromosomal genes in Salmonella. Proc Natl Acad Sci USA 2001, 98:15264–15269.CrossRefPubMed 51. Croes C, Van BE, DeClercq E, Eyers M, Vanderleyden J, Michiels K: Identification and mapping of loci involved in motility, adsorption to wheat roots, colony morphology, and growth in minimal medium on the Azospirillum brasilense Sp7 90-MDa plasmid. Plasmid

1991, 26:83–93.CrossRefPubMed 52. Donnenberg MS, Kaper JB: Construction of an eae Fenbendazole deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect Immun 1991, 59:4310–4317.PubMed 53. Gutierrez C, Devedjian JC: A Plasmid Facilitating Invitro Construction of Phoa-Gene Fusions in Escherichia-Coli. Nucleic Acids Research 1989, 17:3999.CrossRefPubMed 54. Chang ACY, Cohen SN: Construction and Characterization of Amplifiable Multicopy Dna Cloning Vehicles Derived from P15A Cryptic Miniplasmid. J Bacteriol 1978, 134:1141–1156.PubMed 55. Dombrecht B, Vanderleyden J, Michiels J: Stable RK2-derived cloning vectors for the analysis of gene expression and gene function in gram-negative bacteria. Mol Plant Microbe Interact 2001, 14:426–430.CrossRefPubMed 56. Daniels C, Vindurampulle C, Morona R: Overexpression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy).