We investigate the binding of flunitrazepam, the IC50 was approximately 10 nm. Thus, we used a 1000-fold higher Higher concentration of diazepam hrleisten on receptor occupancy and a broad inhibitory effect on weight. With regard to the concentrations of the extract, we tested in a range as low as the concentration of diazepam and as high as the L Solubility of the extract. The incubation was started by addition of 1 nM and flunitrazepam min on the ice for 60 min. The reaction was stopped by vacuum filtration, and jak2 inhibitor each filter was washed with 15 ml of cold 10 mM Tris-HCl. The filters were housed individually in polycarbonate-R Hrchen added and 1 ml of scintillation fluid. The radioactivity t was 2100TR using a Packard Tri Carb Flüssigszintillationsz Hlers. The nonspecific binding is determined by addition of 100 M diazepam in the medium in parallel assays. Specific binding was calculated as the difference between total binding and nonspecific binding. Results are expressed as a percentage of specific binding. 2.7. Statistical analysis The obtained Hte maze, marble burying, and field data were analyzed by analysis of variance for independent Analyzed Independent samples of Duncan test, followed, until the total number of ballots in the arm that obtains Hte maze and the number of stool bolus in the open field test, which were analyzed by Kruskal-Wallis ANOVA followed by multiple comparison test. Flunitrazepam binding data was by ANOVA followed by Student-Newman-Keuls 鈥 Test. Statistical significance was set at P 0.05. Data are expressed as mean standard error of the mean. All statistical analyzes were performed using the Statistica software. Third Results 3.1. Effects of acute administration and chronic alcoholic extract of Achillea millefolium L. erh Hten maze The results of acute administration and chronic alcoholic extract of Achillea millefolium L. erh Hten maze are shown in Fig.
First An ANOVA revealed a difference in the percentage of group entries into the open arms with acute and chronic treatment and the percentage of time spent in open arms. Obtained in groups of short-term treatment, Diazepam and Achillea millefolium extract Ht the percentage of entries Excursions in the time spent on open arms compared to vehicle and lower doses of the extract, yarrow. Similar results were observed in chronic experiments: yarrow extract and diazepam increased the percentage of hte should be Bay 43-9006 B-Raf inhibitor scanned in the time spent on open arms relative to the vehicle. Tthe most important conclusions from this study were anxiolyticlike the effects of water-alcohol extract of Achillea millefolium L. validated in animal models of angstl Send drug screening. The binding data showed that not mediate GABA / BDZ mechanisms of these effects. These results erg Complement previous findings in female rats in a conflict process operating system with aw Ssrigen extract of Achillea millefolium L. flowers. As ben the current animal models do not Term deprivation of food or water, or shock, the results also show that the angstl Send effects, such as Achillea millefolium L. were not on Changes in appetitive behavior or pain sensitivity, the false positive operating conditions provide k can.