D with topotecan at 24 h after treatment with neomycin. But was removed after topotecan, the regeneration of hair cells was severely steamed mpft, Even 72 hours after washing of the inhibitor. Interestingly, treatment with topotecan alone were registered for 24 hours Born delayed loss of hair cells, suggesting that topotecan can hair-cell maturation to t Ten. It was not yet determined whether topotecan-dividing cells, newly formed hair cells or both T Tet. Flubendazole arrests cell division in M phase before the literature suggests that inhibiting flubendazole k Can in microtubule assembly and function after they entered Born in a cell cycle arrest. To test this idea, the fish were controlled groups And the neomycin-treated flubendazole were exposed for 24 h, then immungef Rbt with phospho-histone H3 antibody Body, a marker of proliferating cells. W While cells with an antique Rpern were labeled with PH3 rare in the control groups, we found a significant increase in the number of cells in M phase with flubendazole. Fish exposed to flubendazole in the absence of cell death induced by neomycin hair cells also showed pronounced much Gter with anti-PH3, likely putting an end to the dividing cells in the normal growth process neuromasts. It should be noted that this effect was not limited by flubendazole neuromasts, because there are more anti-PH3 labeled cells along the K Rpers of whole fish after treatment flubendazole. If the BL skirts flubendazole regeneration of hair cells by cell cycle arrest, we hypothesized that this is only effectively blocking the regeneration, when the larvae were treated in the H Highlight of the increase. Characterization of the proliferative events w While showing the regeneration of hair cells in zebrafish a temporary increase P2X Receptor in supporting cells entering S phase and reached 12 to 21 h after neomycin-induced cell death by the hair, and a maximum in cells in M- third phase occurs June h sp Ter.
Therefore groups of fish with the vehicle neomycin flubendazole or DMSO alone treated for 0 or 14, 14 28, 28 or 42 hours after treatment with neomycin exposed. All larvae were collected 48 hours after exposure to neomycin and immunogef rbt For ciliated cells. As shown in Figure 10B, the number of hair cells only in fish with flubendazole 14 to 28 h treatment after treatment reduced neomycin, also supported the idea, the delay help Wrestled flubendazole regeneration of hair cells by arresting cells in M phase Discussion We used our own F ability to hair cells of zebrafish lateral line, regenerate to develop an effective audit of the screen through libraries of drugs for new modulators of hair cell regeneration. W While others questioned selected COOLED compounds that have potential to proliferate in the S Mammal-vestibul To influence Ren epithelium, this survey provides the first chemical widescreen directed to the identification of compounds having the regeneration of hair cells to VER . change Two libraries of drug compounds in 1680 led to two compounds activators and inhibitors ofregeneration six, a rate of 0.48% of various Rft modulator success. In addition, we identified 34 chemicals that ototoxic over 48 h of incubation were. The drugs were tested at concentrations between 1 and 35 m. Our experience has shown that there is little toxicity t at these concentrations. We assume that it tested false negative results with the drugs we overlooked connections that have changed the regeneration of hair cells VER.