This section describes the BioMINT™ bioreactor as an alternative for the micropropagation of agaves within the various phases associated with the micropropagation process.In Mexico, wild agaves are essential when it comes to production of alcohol based drinks known as mezcal and pulque. But, the propagation of agave seeds and pups is certainly not adequate to satisfy the nationwide demand. Temporary immersion systems represent an agave micropropagation alternative that lowers the labor force, increases development, and improves the standard of seedlings. The usage of the SETIS™ bioreactor in A. marmorata and A. potatorum improves the multiplication price and permits rooting. Furthermore, this bioreactor lowers the tradition time, labor pool, and reagents required while maintaining high success prices during the acclimatization stage.Stevia rebaudiana Bert. is a plant that contains noncaloric sweeteners highly valued within the food industry. But, there was a top need for propagules to ascertain commercial plantations, while the conventional reproduction types for this types tend to be inefficient. Micropropagation is a technique that enables obtaining many plants and that can be used to meet the demand in the field. Nevertheless, it needs in vitro propagation practices such as for instance short-term immersion methods (rest) to improve yield and reduce production costs. This chapter defines a highly effective protocol when it comes to large-scale micropropagation of S. rebaudiana making use of a TIS.Sugarcane is used to produce sugar, ethanol, and other by-products, it is therefore considered very crucial plants worldwide. Making use of short-term immersion methods for sugarcane micropropagation signifies an alternative solution to cut back the labor pool, increase plant development, and improve plant high quality. Temporary immersion systems are semi-automated bioreactors made for the large-scale propagation of areas, embryos, and organs. These are temporarily subjected in a liquid culture medium under a specific time and immersion frequency. By using this protocol and a short-term immersion bioreactor, it is possible to attain multiplication and rooting. The use of short-term immersion bioreactors gets better the multiplication price therefore the rooting of sugarcane, reducing the tradition time, work force, and reagents required while keeping high survival prices during acclimatization.The cultivation of vanilla (Vanilla planifolia) is of financial interest because vanillin is extracted from the fresh fruits with this species. Vanillin is an all natural flavoring very respected in the grocery store. However, there is a short supply of propagules available for establishing commercial plantations and good-quality plants with phytosanitary official certification. Plant tissue culture signifies a viable option to provide large amounts of healthy plants to vanilla producers. In addition, the usage type III intermediate filament protein short-term immersion methods will allow commercial scale-up in addition to establishment of biofactories aimed at in vitro vanilla propagation. This chapter describes a large-scale micropropagation protocol for vanilla using short-term immersion bioreactors (TIB).Companies dedicated to the large-scale propagation of plant types are called biofactories or agricultural biotechnology companies. Globally, there are many biofactories (large-scale manufacturing) or plant muscle tradition laboratories (minor production) in charge of providing commercial propagules of plants of economic interest. Each biofactory implements technological developments such temporary immersion (TIS) systems that allow them to lessen expenses. This part analyzes a number of the biofactories set up globally, the key plant species propagated, and whether or not they implement the use of TIS.Technological innovation in the design and make of temporary immersion systems (TIS) has increased in past times decade. Innovations have actually included the scale, fitting, and replacement of components, also as manufacturing products. Air replacement by compressor has also been replaced by environment replacement by predetermined tilting/rotation of culture bottles Probiotic product . This design modification aims to raise the biological yield (range propels) stated in these containers and reduce manufacturing prices. But, the operative concept has remained unchanged through time advertise a breeding ground where explant immersions when you look at the culture medium tend to be Selinexor programmable. The changes in the TIS design incorporate pros and cons, creating the performance of just one kind over another. Nonetheless, validation to identify the top kind of TIS is carried out for each plant types. This chapter details different types of temporary immersion available, emphasizing the benefits and disadvantages of every when used for plant micropropagation.Temporary immersion methods (TIS) are technical resources that support plant micropropagation. Provided their high effectiveness when you look at the in vitro propagation of propels, a current objective is always to update the protocols addressing micropropagation in semisolid culture systems to protocols involving TIS. For this end, different parameters have been evaluated, including TIS kinds and styles, immersion times, immersion frequencies, and level of method per explant, among various other faculties.