A two-tailed Student’s t test was applied. Mouse colonization data are expressed as medians of CFU per gram of stool/fecal contents. Two group comparisons were done by Mann-Whitney U test. A p-value < 0.05 was considered statistically significant. Acknowledgements This work was supported by the European Union Sixth Framework Programme ""Approaches to Control multi-resistant Enterococci (ACE): Studies on molecular ecology, horizontal gene transfer, fitness and prevention"" under contract LSHE-CT-2007-037410 Selleck PLX-4720 and ZonMW “”Vaccine-development to combat the emergence of vancomycin-resistant Enterococcus faecium”" project number

0.6100.0008. The authors thank J. Daalhuisen and M. ten Brink for their expert technical assistance and E. Duizer for helpful comments. References 1. Murray BE: Vancomycin-resistant

enterococcal infections. N Engl J Med 2000, 342:710–721.CrossRefPubMed 2. Dautle MP, Ulrich RL, Hughes TA: Typing and subtyping of 83 clinical isolates purified from surgically implanted silicone feeding tubes by random amplified polymorphic DNA amplification. J Clin Microbiol 2002, 40:414–421.CrossRefPubMed 3. Edmond MB, Ober JF, Dawson JD, Weinbaum DL, Wenzel RP: Vancomycin-resistant enterococcal bacteremia: natural history and attributable mortality. Clin Infect Dis 1996, 23:1234–1239.PubMed 4. Giannitsioti E, Skiadas I, Antoniadou A, Tsiodras S, Kanavos K, Triantafyllidi H, Giamarellou H: Nosocomial BIBW2992 purchase vs. community-acquired infective endocarditis in Greece: changing epidemiological profile and mortality risk. Clin Microbiol Infect 2007, 13:763–769.CrossRefPubMed 5. Leung JW, Liu YL, Desta TD, Libby ED, Inciardi JF, Lam K: In vitro evaluation of antibiotic prophylaxis in the prevention of biliary stent blockage. Gastrointest Endosc 2000, 51:296–303.CrossRefPubMed 6. McDonald JR, Olaison L, Anderson DJ, Hoen B, Miro Tenofovir molecular weight JM, Eykyn S, Abrutyn E, Fowler VG Jr,

Habib G, Selton-Suty C, Pappas PA, Cabell CH, Corey GR, Marco F, Sexton DJ: Enterococcal endocarditis: 107 cases from the international collaboration on endocarditis merged database. Am J Med 2005, 118:759–766.CrossRefPubMed 7. Morrison AJ Jr, Wenzel RP: Nosocomial urinary tract infections due to Enterococcus . Ten years’ experience at a university hospital. Arch Intern Med 1986, 146:1549–1551.CrossRefPubMed 8. Mylonakis E, Calderwood SB: Infective endocarditis in adults. N Engl J Med 2001, 345:1318–1330.CrossRefPubMed 9. Sabbuba N, Hughes G, Stickler DJ: The migration of Proteus mirabilis and other urinary tract pathogens over Foley catheters. BJU Int 2002, 89:55–60.CrossRefPubMed 10. Svanborg C, Godaly G: Bacterial virulence in urinary tract infection. Infect Dis Clin North Am 1997, 11:513–529.CrossRefPubMed 11. Tannock GW, Cook G: Enterococci as members of the intestinal microflora of humans. The enterococci: pathogenesis, molecular biology, and antibiotic resistance (Edited by: Gilmore MS, Clewell DB, Courvalin P, Dunny GM, Murray BE, Rice LBe). Washington, D.C.