1-IDO cells. Murine model of gastric cancer was established. Tumor-bearing mice was divided into 6 gmups: MFC non-transfected group (Group A), pcDNA3.1 group (Group
B), pcDNA3.1-IDO group (Group C), 1-MT-pcDNA3.1-IDO treatment group (Group D), FOLFOX4-pcDNA3.1-IDO treatment group (Group E) and FOLFOX4+1-MT-pcDNA3.1-IDO treatment group (Group F). We can observe the mice tumor cases, differences of tumor weight, and then detect the expression of IDO PD0325901 supplier in tumor tissues by immunohistochemical. The method of RT-PCR was used to detect the expression of IDOmRNA of the spleen organization of tumor-bearing mice. The method of Flow cytometry was used to detect the expression of CD11c, and CD86, and CD80, and Major histocompatibility Antigen complex II (MHC-II) in the surface of Dendritic Cells of the spleen of tumor-bearing mice. Results: Compared learn more with Group A and Group B, the tumor
on Group C grows faster, the weight of tumor increases significantly (P < 0.05). Compared with Group C, the weight of tumor on Group D, Group E and Group F decreases significantly (P < 0.05). The tumor inhibitory rates were 8.91%, 80.20%, 86.13% respectively. There were significant differences between them (P < 0.05), Compared with Group D and Group E, the weight of tumor on Group F decreases significantly (P < 0.05). IDO expression in tumor tissues: Treatment group, cancer cells were less color, especially in the combination group there was even no brown color cells; the gastric selleck kinase inhibitor cancer cells in control group were more, which were arranged in sheets, much larger, colored extensively, and more colored granules. Compared with Group A and Group
B, the expression of IDOmRNA of Group C increases significantly (P < 0.05). The expression of IDOmRNA of Group A and Group B are not significantly different (P > 0.05). Compared with the Group C, the expression of IDOmRNA of group D, group E and group F reduces significantly (P < 0.05). Compared with Group F, the expression of IDOmRNA of Group D and Group E increases significantly (P < 0.05). Compared with the Group C, the number of DC in the spleen of Group A, Group B and Group D increases Slightly. Compared with the Group C, the number of DC in the spleen of Group D and GroupE increases significantly (P < 0.05). Compared with the Group F, the number of DC in the spleen of Group D and Group E reduces Slightly. Compared with the Group C, the surface molecule including CD86, CD80 and MHC-II of DC of the spleen of Group A, Group B, Group D, Group E and Group F increases significantly (P < 0.05). Compared with the Group F, the surface molecule including CD86, CD80 and MHC-II of DC of the spleen of Group D and Group E reduces significantly (P < 0.05).