Experimental T heads Not develop. This turned out to be He his advantage as a large as some of the rhizomes, especially those of the variant Conavit grew up not produce seedlings. This is likely the adverse A-674563 Akt inhibitor effect of humic acid on the growth of fine roots. The dormant rhizomes are sp Ter against S Mling T Heads of perlite mature replaced. S seedlings Cultured before further grown outdoors, independently Ngig on the nature of the substrate, they were transplanted. After three months, R. × bohemica plants were well established and Wei Clover seeds were added to 10 of 20 T Heads of each variant. The F Ability to germinate the seeds was before sowing has been evaluated and found to be 57% based on the average of 10 bo Your Petri, each with 25 seeds. There are about 500 seeds in a gram.
After the first season the plants were harvested in September 2006. We ma S branch numbers and lengths and dry weights and two Reynoutria Mellilotus and 100 mm cut out segments Wurzelst CKE new, Which is formed along the ADX-47273 851881-60-2 wall of the pot, for chemical analysis. The ramification of the branches has also been taken into account, the L Lengths of all the main branches of the h Higher land, and the L Lengths of all sides Residents, measured and evaluated. Fine roots were sampled, w While the roots were separated by hand, wild clover roots, and both were stained and inspected for the presence of mycorrhizae. The experiment was terminated after the second season in September 2007. At the end of the experiment, both above and below ground biomass were measured for the fine roots were mycorrhizal and big e roots and rhizomes were washed thoroughly scanned with the pressure of air and water.
They were then dried and ground for analysis. Clover could grow without RESTRICTIONS LIMITATION in the first season, but the plants were repeatedly cut in the second season, a H He keep 30 cm. The experience in the middle of the 1 ha cloudy with ssert experimental area is not spoiled in one place ° 50 35 N, 13 ° 52 E. This field experience is a former bank, the plow in a field arable and organic fertilizer was converted and still shows a high clay content. In April 2006, 15 20 cm long rhizomes from bohemica R. × were grown at a distance of 70 cm and 100 × were planted immediately covered with soil. Ten plants were taken randomly on each sampling day in July and September 2006, and in May, July and September 2007 and 2008.
The plants were then washed and the surface Surface and underground biomass was measured. Six samples of each series were analyzed for the same stilbenes and emodin as samples from the pot experiment. The biological analysis of stilbenes resveratrol, piceatannol and its glycosides were analyzed terich with emodin in samples of rhizomes and roots of Vogelkn. The soil samples were dried and finely extracted with 60% ethanol, and the extracts were analyzed by HPLC. Fig. Figure 13 shows a typical case of emodin and stilbene measured with this method. Rbeverfahren evaluation of mycorrhizal A modification of a common mycological F Was used between samples to L And stains. Soil samples were rinsed with water through a sieve. Handseparated roots were washed in segments 1 cm 2, with a L Solution of 10% KOH and cut with 0.05% trypan blue in lactoglycerol. Root segments were examined under a microscope at 100 or × × an enlargement TION of 200 and were tested for mycorrhizal colonization. The p