mRNA for TARC and MDC The final set of experiments was carried out to exam-ine the influence of FEX on expression of mRNA for TARC and MDC in C 4 cells after Cry j stimulation. C 4 cells were stimulated with ng/ml Cry j in the presence Nilotinib of various concentrations of FEX for 8 h. The level of mRNA expression was measured by real-time RT-PCR. The addition of FEX to cell cultures at more than ng/ml caused significant suppression of TARC and MDC mRNA expressi which had been enhanced by Cry j stimulation . Discussion Antihistamines such as F A KET and OXA are widely used for treating allergic diseases with remarkable success. The primary pharmacological target of these agents is generally accepted to be the histamine H recep-t with the goal of inhibiting chemical mediator release from Synephrine Alpha-1 receptor inhibitor mast cells and eosinophils.
It has also been reported that these agents suppress the expression of costimula-tory molecules on dendritic cells and the secretion of inflammatory cytokines from T cells and dendritic cells . Howev the influence of antihistamines Nattokinase 133876-92-3 on the production of chemokin which attract T T cells to allergic inflammatory sit is not well defined . Therefo in the present study we examined the influ-ence of antihistamines on TARC and MDC production from C 4 cells in vitro. Our results clearly show that second-generation antihistamin such as A F KET and O but not a first-generation antihistami C suppress the ability of C 4 cells to produce TARC and MDC induced by stimulation with specific antigens and histamine without changes in cell prolifera-tion by antigenic stimulation.
We also demonstrated that FEX is the most effective agent in terms of the suppres-sion of TARC and MDC production from C 4 cells after Cry j stimulation. This conclusion may be sup-ported by the observation that FEX exerted a significant 6 Int Arch Allergy Immuno. Shoji /Asano /Furuta /Hirano /Suzaki TARC levels TARC levels Not detected Not buy Cytisine detected Not detected Not detected TARC levels TARC levels Not detected Not detected Not detected Not detected Med. PPD Med. PPD Fig. 0. Influence of antihistamines on the a 0 alone alone PPD FEX b 0 alone alone PPD AZE production of MDC from C 4 cells in response to PPD stimulation in vitro. C 4 cells from nonallergic subjects were stimulated with 0 g/ml PPD in the presence of various concentrations of the antihistamines FE AZ KET and OXA for days.
MDC levels in cul-ture supernatants were examined by ELI-SA. The data are expressed as means SE. Med. PPD . Med. PPD 0 Med. = Medium. p ! versus PPD c alone alone PPD KET d alone alone PPD OXA alone. suppressive effect at a concentration of ng/ which is almost equal to therapeutic blood levels , but other agen A KET occupation and O required higher concentra-tions than their ther-apeutic blood levels . TARC and MDC are T T cell chemokines that bind and attract CC chemokine receptor -positive T -type T cells . It was reported that serum obtained from patients with atopic dermatitis contained much higher levels of both TARC and MDC than that from normal subjects and that these chemokine levels were significantly correlated with the severity of these atopic diseases . In the case of allergic rhinit nasal epithelial cells from patients with pollinosis released much higher levels of TARC.