A yeast-containing phagosome Embrane, followed by the assembly of actin and exocytosis. The cells expressing GFP and MRFP VATM whitewashed. Found at: doi: 10.1371/journal.pone.0008585.s009 movie S6 L mixture of BMS-599626 HER2 inhibitor the V-ATPase from the membrane of a phagosome, the yeast, followed by the assembly of actin and exocytosis. During the same interval, the cell takes place in a new yeast. The cells expressing GFP and MRFP VATM whitewashed. Found at: doi: Movie S7 10.1371/journal.pone.0008585.s010 exocytosis of a yeast-FITC from a phagosome whose membrane was publ before exocytosis of GFP VATM pft. The cells expressing GFP treated with lime and VATM DdmCherry. The yeast is not always bright when they come into contact with the extracellular Ren medium. Found at: doi: 10.1371/journal.pone.0008585.
s011 movie S8 premature exocytosis of yeast contains lt phagosome and early phase of recovery of GFP VATM the plasma membrane. The cell, the GFP and tubulin VATM mRFPa. Found at: doi: 10.1371/journal.pone.0008585.s012 movie S9 separation of large vacuoles s V-ATPase-rich yeast, the premature exocytosis before phagosome. The cell is expressing GFP and MRFP Vismodegib 879085-55-9 VATM tubulin. Found at: doi: S Movie S10 10.1371/journal.pone.0008585.s013 acid nature of the early phagosome shows exocytosis, and actin movement driven vacuole. The cells expressing GFP treated with lime and VATM DdmCherry. He a an FITC-labeled yeast, which is hardly visible in the phagosome, but brighter when the extracellular Ren pH affected, indicating that the light was the acidic phagosome. Found at: doi: S11 10.1371/journal.pone.
0008585.s014 movie premature exocytosis of a phagosome with a yeast-FITC and dynamics of the vacuole that separates prior to the removal of the V-ATPase PLoS ONE | Published in PloSOne 12th January 2010 | Volume 5 | Number 1 | e8585 exocytosis. The cells expressing GFP treated with lime and VATM DdmCherry. Found at: doi: 10.1371/journal.pone.0008585.s015 movie S12 increase the volume of the phagosome and the dilution of the fluid phase marker before premature exocytosis. The cell is expressing GFP VATM and was incubated for 3 h with TRITC-dextran and then moved to the buffer and unlabeled observed. Found at: doi: 10.1371/journal.pone.0008585.s016 film acquisition of GFP S13 2FYVE label of a vacuole, the phagosome separated shortly before a premature exocytosis.
The cells expressing GFP and MRFP 2FYVE GE Cares, the phagosome with a yeast-FITC. Found at: doi: 10.1371/journal.pone.0008585.s017 Acknowledgements We thank Sergio Grinstein for helpful comments and for the gift of plasmid 2FYVE pEGFPC1, Jody Gross for the transfer of the relevant portion of this plasmid with an expression vector Dictyostelium, Margaret Titus for MYOB GFP plasmid, and Widmar Tanner for the yeast strain TH2 1B. We recognize the material and technical support from the Fund for Basic Imaging at Oklahoma Medical Research Foundation and the Nikon Imaging Center at the University Of Heidelberg. Author Jaworek Con U and developed experiments: MC. The experiments carried out MC. Data Analysis: EU MC GG. Post reagents, equipment used and analytical tools: LM MC EU. The paper wrote: MC GG.
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