Boca Raton, Florida: CRC Press, Taylor & Francis Group; 2006:247–263.CrossRef 23. Alma A, Daffonchio D, Gonella E, Raddadi N: Microbial Symbionts of Auchenorrhyncha transmitting phytoplasmas: a resource for symbiotic control of phytoplasmoses. In Phytoplasmas: Genomes, Plant Hosts and Vectors. Edited by: Weintraub P. and Jones P. Wallingford: CAB International; 2010:272–292. 24. Favia G, Ricci I, Marzorati M, Negri I, Alma A, Sacchi L, Bandi C, Daffonchio D: Bacteria of the genus Asaia : A potential weapon against malaria. Adv Exp Med Biol 2008, 627:49–59.PubMedCrossRef 25. Sacchi L, Genchi

M, Clementi E, Bigliardi E, Avanzati AM, Pajoro M, Negri I, Marzorati M, Gonella E, Alma A, Daffonchio D, Bandi C: Multiple symbiosis in the leafhopper Scaphoideus titanus (Hemiptera: Cicadellidae): details of transovarial transmission of Cardinium sp. and yeast-like endosymbionts. Tissue Cell PD-0332991 cell line 2008, 40:231–242.PubMedCrossRef 26. Lambertsen L, Sternberg C, Molin S: Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins. Environ Microbiol 2004, 6:726–732.PubMedCrossRef 27. Moutous G, Fos A: Essais de rhizogénèse chez la feuille de vigne isolée. Revue de Zoologie Agricole et de Pathologie végétale 1973, 27–28. 28. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Z-VAD-FMK concentration Manual. 2nd edition. Cold

Spring Rho Harbor: Cold Spring Harbor Laboratory Press; 1989. 29. Raddadi N, Gonella E, Camerota C, Pizzinat A, Tedeschi R, Crotti E, Mandrioli M, Bianco PA, Daffonchio D, Alma A: ‘ Candidatus Liberibacter europaeus’ sp. nov. that is associated with and transmitted by the psyllid Cacopsylla pyri apparently HKI-272 nmr behaves as an endophyte rather than a pathogen. Environ Microbiol 2011, 13:414–426.PubMedCrossRef 30. Li J, McLellan S, Ogawa S: Accumulation and fate of green fluorescent labeled Escherichia coli in laboratory-scale drinking water biofilters. Water Res 2006, 40:3023–3028.PubMedCrossRef 31. Marzachì C, Bosco D: Relative quantification

of chrysanthemum yellows (16Sr I) phytoplasma in its plant and insect host using Real Time PCR. Mol Biotechnol 2005, 30:117–127.PubMedCrossRef 32. Fuchs BM, Wallner G, Beisker W, Schwippl I, Ludwig W, Amann R: Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes. Appl Environ Microbiol 1998, 42:4973–4982. Competing interests The authors declare that they have no competing interests.”
“Background Bacterial intracellular symbiosis (endosymbiosis) is widespread in invertebrates and exhibits a large variety of phenotypes, ranging from mutualism to pathogenesis. Endosymbionts are transmitted vertically for hundreds of host generations and affect the host biology in many ways, including reproduction, physiology and behavior [1–4].