0.05 5.00 L mol L. Treatment was initiated by suspending embryos in diluted L Measurements. at the end of treatment, the embryos were rinsed three times with MFL, using a centrifuge by hand. Z choose The number of pigment cells BRL-15572 of the pigment cells was obtained by the method described by Kominami.

Briefly, the embryos were collected by hand centrifuge, washed twice with double St Ca2 strength artificial seawater washed, and in the same L Solution w min During 20 30. Thus, the treated embryos were fixed in 10% formalin sea water, and were recorded on a Objekttr hunter mounted with a drop of glycerol. After placing a cover glass, the embryos were by the removal of the L Solution with a piece of L Schpapier compressed. Number of pigment cells were obtained on photographic images.
In situ hybridization and HP00367 HP00444 used to prepare RNA probes for the detection of cells and cells blastocoelar number needles are. Former thymosin beta code and its RNA probe detects cells blasotcoelar. It codes for 3 Untranslated region of the gene known, but the RNA probe tip detect cells of needles. RNA probes were synthesized as described Streptozotocin by Ogasawara et al. Whole mount in situ hybridization was performed using the protocol by Shoguchi et al. Zellz Hlung z Lomischen pocket 4 Diamidino phenylindole dihydrochloride 2 was resolved in DMSO at 10 mmol like a camp St. This Stamml Solution was diluted with phosphate-buffered salt solutions Solution to a final concentration of 1 lmol L. The embryos were collected with the L Solution for 30 minutes found Rabbit, and three times with PBS.
The samples were observed under an epifluorescence microscope, and nuclei were z Lomischen pocket through which st YOUR BIDDING Hlt Santander, the firing level gez. In some cases F, The embryos were found under the microscope Rbten confocal laser scanning investigated. Detection of muscle fibers circumstances Ends feeder Lead cancer procedures described by Cline and shadow used to stain muscle fibers circumstances Ends feeder Lead cancer, but a few Changes were made. In short, fixation and F Staining simultaneously have been made in MFL with 5% formalin, 0.5 lg L taxol, 1% Triton X 100 and 40 ng L rhodamine phallocentrism Dine. Rin by lacing samples three times with 5% formalin-sea water, were found Rbte embryos slightly crushed, and examined under green light illumination.
Results Morphology of embryos with DAPT pulcherrimus gastrula embryos treated the sp Hemicentrotus achieved Ter at 24 hours after fertilization. At the head of primitive gut, a series of CML was observed. Even if the embryos were treated continuously with 0.5 L mol DAPT L, they showed little understanding Change in U Ere morphology, au It to expand the base of the intestine. When treated with DAPT Lmol L is the invagination of the gut rudiment much zinc Siege. Extension of the basic primitive gut was also noticed. Cells in filopodia archenteron hardly noticed on the front line, indicating that the differentiation of MSC was blocked hard. The formation of the oral aboral axis in much less than h Higher concentrations of DAPT were interrupted two ventral lateral groups of prime Ren mesenchymal cells were observed. Approx Hr 48 HPF, the embryos developed in the early pluteus, which distributes a number of dendritic pigment cells in the aboral ectoderm. The archenteron is differentiated in a digestive t