T has been discussed in the section were VLB and VCR used as starting points for semisynthetic analogs. In many of these studies, no discussion spectroscopy has been reported only given a list of some of the peaks H properties of synthetic or partially Biosyn, H and C tasks. In some cases F Other NMR Dihydrofolate Reductase spectroscopy served as an indispensable instrument for the possible reaction mechanisms to understand. InSundberg studied and co workers Changes Polonowski Potier coupling reaction catharnathine and vindoline, where they were first isolated and identified a highly reactive iminium intermediate by NMR spectroscopy at low temperatures. This key intermediate helped optimize this reaction, which was used sp Ter in many cases Cases, to produce derivatives of VLB and VCR.
One of the gr Th semi-synthetic derivatives before VLB prepared by this reaction is the already mentioned Hnte vinorelbine. Described as similar to VLB, w Was completely while the C-NMR spectrum of nearly Attributed ndig spec were only fragmentary H-NMR data of Potter et inwhen workers synthesized this compound has been reported. The first completely Requests reference requests getting H and C could assign-ment only performed inwhen L Sen MHz NMR spectrometer and inverse detection techniques het eronuclear Korrelationsma was available. The last semi-synthetic derivative of VLB with a promising potential iCal flashing vinflunine, synthesized from vinorelbine in Supers Acid media. The complete H and C NMR assignment of the base and salt was carried out bitartarate inusing homo and hetero technical D.
additives Tzlich has to these emissions, the conformation of the base in acetone was studied on the basis of NOE data connectivities t, and in analogy to derivatives and other VLB, was closed to have show the same properties as the conformation found in the solid state. Recent research directions syntheticbiological the structural point of view of structural change in addition to VLB, a relatively new field of research is syntheticbiological to different types of bioconjugate VLBVLB analogues using known differences in the biochemical behavior of cells and prepare cancer. To this end, various biomolecules have been pl neck Dierte derivatives for VLB. The structural characterization of these conjugates was primarily to determine the number of bisindole molecules on the antique Bound body and see the references cited Descr Nkt.
Zus Tzlich bound to this antique Rperkonjugate is, small peptides or other molecules st Leaders tubulin were also derivatives of VLB produces folic searches Acid, or conjugated phomopsin thiochicine oligoarginine and hybrids. Sim Similar to the big s molecule associated VLB derivatives, was the main goal of these studies to Ver Changes in biological activity T and specificity of t of these conjugates in bisindoles and techniques to examine the NMR and MS were in the majority only for the F lle, ver Ify, structures, without using their spectroscopic properties. A more detailed spectroscopic study of these hybrids and conjugates was reported in two papers. The first was on phomopsin anhydrovinblastineC VLBvinorelbine hybrid delta, were obtained in this case D and D NMR data atMHz supported by HR ESI-MS data. On the basis of the observed data NOESY