GBT does not offer any advantage in FB patients.”
“Numerous studies recommend collagen to be employed in constructing scaffolds for bone tissue engineering.
The aim of this study was to test whether addition of hyaluronic acid (HA) and of human bone morphogenetic protein 4 (BMP-4) to collagen scaffolds improves its physical-chemical and morphological properties and biocompatibility with human MG 63 osteoblast-like cells. Type I fibrillar collagen, HA and BMP-4 were used to prepare 3D porous scaffolds by freeze-drying method. The structure of these natural polymers was stabilized employing 0.25% glutaraldehyde, a method that warrants that all the scaffolds components keep their structural integrity and biological properties. The physical-chemical CDK inhibition properties were assessed using infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and thermogravimetry (TG/DTG); the morphological properties were evaluated by scanning electron microscopy (SEM) and water uptake. The biological properties were determined (in vitro) by collagenase degradation and by assessment of the biocompatibility with MG 63 osteoblast-like cells. The results demonstrated that the collagen scaffolds with HA and BMP-4 had good physical-chemical and morphological properties, promoted adhesion, maintained viability and sustained the migration of osteoblast-like cells into scaffolds.”
“The main objective of the study
was to compare the effects of selleck chemicals PX-478 short-duration pH treatments on root hydraulic properties in trembling aspen (Populus tremuloides) seedlings that were either inoculated with the ectomycorrhizal fungus Hebeloma crustuliniforme or remained non-inoculated (control). Inoculated and non-inoculated plants were exposed in solution culture to the root zone pH ranging from 4 to 9 and their root
hydraulic conductivity was examined using the hydrostatic method and after subjecting the plants to treatments with 100 mu M HgCl(2) (aquaporin blocker) and 0.02% trisodium 3-hydroxy-5,8,10-pyrenetrisulfonic acid (apoplastic transport tracer). In a separate experiment, pure cultures of H. crustuliniforme were also grown on a slid medium with the pH ranging from 4 to 9 to determine their pH growth optimum and changes in medium pH over time in the presence and absence of 8 mM NH(4)NO(3). When grown in pure culture, H. crustuliniforme demonstrated maximum growth at pH 7-8 and was capable of modifying the pH of its growth media, especially in the presence of NH(4)NO(3). The plants that were inoculated with H. crustuliniforme had a maximum root hydraulic conductivity at pH 7. At this pH, root hydraulic conductivity was significantly higher compared with non-inoculated plants and showed greater sensitivity of root water transport to pH changes relative to non-inoculated seedlings. Relative apoplastic flux was largely unaffected by pH in inoculated seedlings.