Histamine-mediated signals affect the ability of DC to induce the maturation of T cells along Th1 or Th2 pathways. Histamine appears to be involved in the Th-switch: Th1 cells express H1R, while H2R is found on both Th1 and Th2 cells, as well as DC. H2R also appears to play a critical role in the induction of immune tolerance. Histamine has many important, but still poorly understood immune-related functions, highlighting the need for additional animal models, including histamine receptor gene knockout

mice. Mast cells play critical, but undefined, immunoprotective roles in bacterial and helminth BMS-354825 cell line infections. Studies from the laboratory of Richard Stevens (Boston, MA) led to the identification of two major serine mast cell tryptases, mouse mast cell protease (mMCP)-6 and mMCP-7, that are critical factors in protection from bacterial and helminth infection. Dr. Stevens and colleagues demonstrated that mast cell-deficient

W/Wv mice can successfully combat a Klebsiella pneumoniae pulmonary infection when pre-treated with physiologic amounts of recombinant mMCP-6 or its human ortholog hTryptase-β 17. Dr. Stevens and Dr. Adachi created transgenic mice that lack both mMCP-6 and mMCP-7 18. They then showed that these tryptase-deficient animals have a markedly reduced ability to combat K. pneumoniae infection of the peritoneal cavity and an impaired ability to combat Trichinella spiralis infections. The mechanisms by which mast cell-restricted tryptases INK 128 cost are beneficial in varied infections remain to be determined at the molecular level, but it appears that they play important roles in orchestrating the accumulation of granulocytes in tissues. K. Frank Austen (Boston, MA) addressed an unexpected

role of mast cell proteases in the response to ischemia reperfusion injury. In mouse models of ischemia reperfusion injury, the heightened exposure of self-Ag leads to Ag recognition by natural IgM and subsequent complement activation. This results in immune mediated injury that depends on specific mast cell-derived proteases, as evidenced by the fact that mast cell-deficient mice are protected from injury. In hind limb ischemia reperfusion injury, mice Rebamipide lacking the elastase mMCP-5 are significantly protected. The same mechanistic principles apply to a second-degree burn model in which mice deficient in mast cell chymase/elastase (mMCP-4/5), but not tryptase (mMCP-6/7), are protected from ulceration and scarring. Dr. Austen proposes that mast cell-derived proteases such as mMCP-4/5 play a critical role in the tissue damage following injury. Stephan C. Bischoff (Stuttgart, Germany) observed that much of the mast cell literature is based on data obtained in animal species that, in nature, do not suffer from mast cell-mediated allergic diseases.