Inclusion complexes between trans-resveratrol and ��-cyclodextrin or hydroxypropyl-��-cyclodextrin revealed improved solubility and kept the scavenging capacity against the stable radical DPPH? [19]. Resveratrol-loaded lipid-core nanocapsules increased the concentration of trans-resveratrol in the brain tissue and can be used for several brain diseases including Alzheimer’s selleck Abiraterone disease [20]. Pharmacokinetic studies of resveratrol-loaded nanoparticles were performed and demonstrated an increased systemic bioavailability [21]. Vanillin cross-linked chitosan microparticles showed a controlled release of resveratrol [22]. Inspite of these papers, the literature does not report studies involving resveratrol-loaded microparticles obtained from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(��-caprolactone) (PCL) as polymers.

These polyesters are attractive materials for controlled-release drug applications due to their biodegradability. Their decomposition products are not toxic and are easily excreted [23]. Moreover, a lack of data is available regarding the effect of resveratrol-loaded microparticles on hemolysis of red blood cells. Thus, the aim of this paper was to obtain resveratrol-loaded PHBV/PCL microparticles by simple emulsion/solvent evaporation method and to evaluate their erythrocyte cytotoxicity using the hemolysis assay in order to investigate the feasibility of applying these polyester microparticles as an oral drug delivery carrier for controlled release.2. Materials and Methods2.1. Materialstrans-resveratrol (99.

8% pure, Pharma Nostra, Rio de Janeiro, Brazil), poly(3-hidroxybutirate-co-3-hidroxyvalerate) (PHBV) (Mw? = 380,000g?mol?1, 8.70mol% hydroxyvalerate, Biocycle L110, PHB Industrial, Serrana, Brazil), poly(��-caprolactone) (PCL) (Mw? = 70,000�C90,000g?mol?1, Sigma-Aldrich, St. Louis, MO, USA), and poly(vinyl alcohol) (PVA) (Mw? = 72,000g?mol?1, 88.5mol% of hydrolysis, Vetec, Rio de Janeiro, Brazil) were used as received. The other reagents and solvents were of analytical grade.2.2. Preparation of Resveratrol-Loaded PHBV/PCL MicroparticlesThe polyester microparticles Carfilzomib containing resveratrol were prepared by simple emulsion/solvent evaporation procedure [23]. Three different formulations (Table 1) were obtained for each polymer (PHBV/PCL) depending on the amount of resveratrol into their compositions (5, 10, and 20%). Chloroform and methylene chloride were used as polymer solvent for PHBV and PCL, respectively. Briefly, the organic phase was added into the aqueous phase under mechanical stirring (5.000rev?min?1) for 5min. The emulsion was kept under mechanical stirring (800rev?min?1) at room temperature for 4h.