Of the 11 sites with positive detection in common with the 1992–1994 survey, Slackwater Darter was detected at five sites (all breeding sites), suggesting a 45 % reduction in range, typically with a higher number of sampling trips (Table 1). Six of the ten sites with positive detection in this study were breeding sites, while four were samples taken in non-breeding habitat outside of the spawning season
(Appendix). Five of these (2 breeding and 3 non-breeding sites) were novel (e.g., not shared with previous studies). Fig. 2 Sampling sites for Etheostoma boschungi MRT67307 in vivo in the Cypress Creek watershed over time. White circles are sites where the species was not detected; black circles were sites with positive detection, and stars represent new site records for that time period Table 1 Detection of Etheostoma boschungi SB-715992 price by repeated sampling of locations over time Stream and site # 1970s 1992–1994 2001–2013 Cypress Creek system Lindsey, 57a 100 % 0 – Lindsey, 7a 100 % 0 0 n = 6 Lindsey, 4a 100 % 0 0 n = 4 Greenbrier, 29 100 % 0 0 n = 3 Middle Cypress, 28a 100 % 0 0 Burcham, 1 100 % 0 0 Bruton, 2 100 % 0 0 N Fork, 11 100 % 0 0 n = 2 N Fork, 13 100 % 0 0 n = 2 Cemetery Branch, 10 100 % 0 0 Middle Cypress, 25 100 % 100 % n = 3 100 % 10/10 Middle Cypress, 32a – – 100 % 1/1 Elijah Branch, 12
100 % 0 0 Spain Branch, 33a – 100 % 0 Lindsey, 5 – 100 % 0 Cypress Inn, 15 100 % 100 % n = 2 0 Natchez Trace, 20 – 100 % n = 4 25 % 3/12 Little Shoal Creek Little Shoal, 34 – 100 % n = 3 16 % 1/6 Swan Creek Swan, 45a – 100 % n = 10 20 % 1/5 Swan, 40 – 100 % n = 2 0 n = 7 Collier Creek, 39 – 100 % 0 n = 3 Brier Fork Brier Fork, 51 – 100 % n = 2 16 % 1/6
Brier Fork, 52 – 100 % n = 5 0 n = 3 Brier Fork, 49a – – 33 % 1/3 Brier Fork, 54 – – 100 % 1/1 Brier Fork, 50a – – 50 % find more 1/2 Brier Fork, 55 – – 100 % 1/1 Copeland Creek, 56 100 % 100 % 0 n = 2 West Forkb 100 % 0 – Buffalo River Chief Creek, 37 100 % 0 0 n = 2 Only sites with positive detection during one of the three time periods included. Collections based on single sampling effort unless numbers of trips indicated. Fractions indicate number of positive detections over total number of sampling trips. Collections from the 1970s from Wall and Williams (1974) and Boschung (1976, 1979); 1992–94 from McGregor and Shepard (1995), and 2001–13, current study. Site numbers correspond to the Appendix aNon-breeding sites bNot sampled in 2000s Other sites that were shared with the previous survey have detectabilities ranging from 14 to 25 % (Table 1). This contrasts with the survey conducted by McGregor and Shepard (1995), where detectability was 100 %. Slackwater Darters were not detected at other historical sites, however, the species was detected at three sites in the Brier Fork system that were not sampled by McGregor and Shepard (1995) (sites 49, 50 and 55; Fig.