Of the other probes listed in Table 1, ABI1246 was strongly positive with all four Abiotrophia/Granulicatella reference strains tested (Granulicatella adjacens CCUG 27809T and HE-G-R 613A, Granulicatella elegans CCUG 38949T and Abiotrophia defectiva CCUG 36937), whereas ABI161 labeled only the Granulicatella strains. Probe LCC1030 was positive with Lactococcus lactis subsp. lactis reference strain NCC2211 [17], and the S. mutans and S. sobrinus probes Smut590 and L-Lsob440 stained reference strains UA159T and OMZ 176, respectively, while
none of the probes was positive with strains from other streptococcal species. Probe SRT2104 order L-Ssob440-2 yielded better fluorescence intensity than the previously described probe SOB174 [10], but had to be used at high selleck products stringency. All these findings check details were as expected from in silico data. Table 2 Reactivity of FISH probes to lactobacilli with target and non-target strains 16S rRNA probes Group, Strain OMZ LGC358a LAB759 + LABB759-comp Lpla759 Lpla990 + H1018 L-Lbre466-2 L-Lbuc438-2 Lcas467 Lsal574 L-Lsal1113-2 Lreu986 + H1018 Lfer466 + H448+ H484 L-Lcol732-2 Lvag222 Lgas458 Lgas183 L. buchneri et rel. L. plantarum FAM 1638
945 2-4+*,a 3-4+ 3-4+ 2-4+* – - – - – - – - – - – L. brevis ATCC 14869 625 3-4 + 2-3 + – - 4+ – - – - – ± -b – - – L. brevis OMZ 1114 1114 2-4+ 2-3+* – - 3-4+ – - – - – - -b – - – L. buchneri ATCC 4005 626 2-4 + 1-2 + – - – 3-4 + – - – - – -b – - – L. buchneri 1097 2-4 +* 2-3 +* – - – 3+ – - – - – -b – - – L. casei et rel. L. casei ATCC 393 939 2-4+ 3-4+ – - – -c 3+ – - – - – - – - L. casei Cl-16 638 3-4 + 3-4 + – - – -c 3-4 + – - – - – - – - L. paracasei ATCC 25598 624 2-4 +* 2-4 +* – - – -c 3-4 +* – - – - – - – - L. rhamnosus AC 413 629 2-4 + 2-4 + – - – - 3-4 + – - – - – - – - L. rhamnosus ATCC 7469T 602 2-4 + 2-4 + – - – - DOCK10 3 + – - – - – - – - L. salivarius L. salivarius ATCC 11741 525 3-4+ 3-4+ – - – - – 2-4+ 3-4+ ± – - – - – L. salivarius OMZ 1115 1115 2-4+ – - – - – - 3-4+ 3-4+ – - – - – -
L. reuteri et rel. L. coleohominis DSM14060T 1113 1-3 + 2-4 + – - -d – - – - 3 + – 3-4 + – - – L. fermentum ATCC 14931 524 2-4 +* 2 +*, e – - – - – - – 2-4 + 3-4 + – - – - L. fermentum OMZ 1116 1116 2-4 + 2 +*, e – - – - – - – 2-4 + 3-4 + – - – - L. reuteri CCUG 33624T 1100 2-4 + 3-4 + – - – -c ± – - 2-4 + 2-4 + – - – - L. vaginalis UMCG 5837 1095 2-4 + 3-4 + – - – -c – - – 1-3 +* – - 3-4 + – - L. gasseri et rel. L. acidophilus ATCC 4357 523 2-4+ 3-4+ – - – - – - – ± ± – - 2-4+ – L. crispatus ATCC 33820 522 3-4 + 3-4 + – - – - – - – - – - 3-4 + – L. gasseri ATCC 19992 520 2-4 + 2-4 + – - – - – - – ± 1 + – - 1-3 + 2-4 + L.