Methods Bacterial strains 331 invasive isolates of Streptococcus pneumoniae

from the Health Protection Agency collection, London, UK, collected during the period 2002–2006, were selected among the 10 major MLST sequence types (STs), circulating in England and Wales (see [27] and [28] for detailed MLST methodology), with approximately 30 isolates per ST. Selection included serotypes commonly associated with these STs and all possible serotype variants www.selleckchem.com/products/BI6727-Volasertib.html (Table 1) identified in the HPA collection. Isolates were serotyped by slide agglutination against the full antisera panel from the Danish Statens Serum Institute (Denmark) as part of the Systemic and Respiratory Infection Laboratory (HPA, London) learn more reference service. The isolates were collected from blood (314), cerebral spin fluid (13), pleural fluid (2), abscess (1), and bronchial aspirate (1). Table 1 Distribution of the 331 S. pneumoniae isolates Sequence Types (ST) Serotypes Number see more of Isolates Singletons MLVA Types (MT) Clonal Complexes (CC)     Per Serotype Total       9 8 1 33 0 199 CC8 19 F 2   0 228, 182 14 30   0 199, 304, 375, 280, 337, 272, 367, 350, 365,, 354, 347,

383, 378 65 18C 1 33 0 209 CC5 22A 1   0 172, 6A 29   0 172, 334, 271, 324, 314, 177, 176, 173, 358, 363, 257 6B 2   0 172, 206 138 6B 30 30 5 234, 330, 276, 251, 268 CC1 311, 345, 310, 326, 289, 181, 369, 319, 344, 370, 315, 273, 246, 295, 254, 269, 356, 233, 213, 237,

210, 290, 329 156 14 4 34 0 299, 249, 190, 229 CC9 6B 1   0 207 9 V 29   1 179, 184, 204, 192, 343, 168, 188, 187, 169, 277, 198, 113, 328, 352, 171, 200, 278, 279, 183, 189, 205, 174, 175, 287 162 MC162a 1 1 33 0 364 CC9 14 1   0 225 19 F 14   3 270, 300, 301, 325, 368, 239, 309, 323, 372, 348, 374, 346, 341 MC162b 9 V 4   2 263, 281, 252 CC10 19 F 1   0 351 6B 1   0 227 9 V 11   0 265, 306, 245, 258, 293, 336 176 6B 3 31 0 193, 377 CC11   27   3 282, 274, 214, 371, 307, 224, 219, 338, 178, 313, 305, 262, 170, 361, 267, 266, 340, 335, 185, 196, 253, 236, 217, 308 CC4 6A 1   0 224 CC4 180 14 1 33 0 129 CC7 19 F 1   0 223 3 30   2 331, 296, 288, 156, 232, 138, 285, 238, 222, 384, 312, 248, 327, 349, 360, 230, ID-8 294, 240, 318, 320 7 F 1   0 222 199 15B 7 42 1 180, 191, 194, 256, 195, 216, 220 CC6 15C 4   1 243, 366, 362 19A 29   0 235, 283, 231, 203, 297, 316, 302, 317, 376, 292, 379, 333, 359, 242, 259, 244, 355, 284, 260, 339 19 F 1   0 197 9 N 1   0 203 227 1 29 32 0 215, 380, 250, 255, 186, 211, 201, 322, 298, 247, 357, 342, 376, 264, 382, 275 CC3 14 1   0 186 6A 2   0 211, 226 306 1 29 29 0 85, 261, 130, 353, 212, 241, 221, 202, 303, 381 CC2 Bold MTs are referring to singletons. Methods MLVA was performed as previously described [23]. The first 17 VNTRs (Spneu 15 to Spneu 41) were used. The last one (Spneu42) unsuccessfully amplified DNA from the isolates or the reference strains and therefore was avoid in this study.