Results are the average of the motility zones of sixteen Petri dishes per strain. Data was statistically analyzed using one-way ANOVA (p < 0.05). Acknowledgements We thank Rodrigo Vena for assistance with the confocal microscopy Afatinib cost facility, Microquin for the culture media, Catalina Anderson (INTA Concordia, Argentina), Gastón Alanis and Rubén Díaz Vélez (Proyecto El Alambrado) for the citrus plants, Sebastián Graziati and Diego Aguirre for plant technical

assistance and the Proteomics laboratory from the Biosciences core laboratory, King Abdullah University of Science and Technology, for providing the facility and equipment for gel electrophoresis and mass selleck chemicals llc spectrometry analyses. This work was supported by grants from the Argentine Federal Government: ANPCyT (PICT2010-1507 to NG and PICT2010-0300 to JO) and CONICET (PIP2010-2012 to JO and NG), the Fundación Josefina Prats to CGG and FAF. JO and NG are staff members and TZ, GGS, CGG and FAF are fellows of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina). Electronic supplementary material Additional file 1: Figure

S1: Characterization of the hrpB − complemented strain on HR and pathogenicity. (A) Schematic organization of the hrp cluster of X. citri that was constructed based on the X. citri subsp. citri strain 306 genome sequence [1]. Boxes correspond to ORFs, arrows Niraparib price indicate orientation of the hrp operons. The hrp, hpa and hrc genes are indicated. Dotted boxes indicated the genomic regions replaced by mutagenesis. Bellow of the scheme, the black box represents the genomic fragment cloned in pBBR1MCS-5 to complement the hrpB − mutant strain. (B) Bacterial suspensions of X. citri,

the hrpB − mutant and the hrpB − c strains were inoculated at 108 CFU/ml into the intercellular spaces of fully expanded tomato, cotton and pepper leaves. A representative photograph of a leaf is shown after 1 day of inoculation. (C) As in B, bacterial suspensions at 107 CFU/ml were inoculated into the intercellular spaces of fully expanded citrus leaves. A representative photograph of a leaf is shown after 8 days of inoculation. (D) RT-qPCR to determine Low-density-lipoprotein receptor kinase CsLOB1 expression levels in leaves after 48 hours of infection with X. citri, the hrpB − mutant and hrpB − c strain. Bars indicate the expression levels relative to buffer infiltrations. Values are the means of four biological replicates with three technical replicates each. (PDF 137 KB) Additional file 2: Figure S2: Swimming and swarming assays. Representative photographs of Petri dishes with X. citri, the hrp mutants and the hrpB − c strain after 2 days of inoculation. Scale bars: 10 mm. (PDF 819 KB) Additional file 3: Table S1: Oligonucleotides used in RT-qPCR assays. (PDF 7 KB) References 1.