Statistical analysis Paired and unpaired t tests were performed using GraphPad Prism version 6. 00 for Mac OS X, in contrast to breast cancer pa selleck chemical 17-AAG tients who showed values between 18 and 108. 54 pgml, a difference which proved to be sta tistically significant. In the group of breast cancer patients, VEGFA initially increased in all patients and declined back on day 7, except for one outlier with locally advanced disease who eventually relapsed. These changes reached a statistical signifi cance on day 3 compared to presurgical values and on day 7 compared to day 3. FGF2 values Inhibitors,Modulators,Libraries were quite similar for both the control and the study group preoperatively and in some cases below the detection limit of the assay. A postsurgical increase was documented in most patients.
This in crease continued on day 7 following surgery for the breast cancer group. However, these changes did not reach statistical significance at any time point. IL8 expression was higher in the study group prior to surgery, with values ranging between 2. Inhibitors,Modulators,Libraries 59 and 33. 7 pgml, a difference that was statistically significant. In breast cancer patients, Inhibitors,Modulators,Libraries IL8 initially showed a slight increase towards day 3, but on day 7 it decreased to levels lower than the preoperative values. Gene expression analysis We performed quantitative RT PCR analysis for 84 angiogenesis related genes in blood from patients with breast cancer and fibroadenoma before surgery. A scatter plot analysis of the results together with a heat map is shown in Figure 3, as well as a comprehensive list of all the changes.
We also analyzed the expression of gene transcripts between patients with cancer and benign breast disease after surgery in an effort to identify possible differences in expression patterns between these two groups of patients as a result of the surgical procedure itself. Re sults are shown in Figure 4. Surgery induced an altered Inhibitors,Modulators,Libraries expression in several keygenes in breast cancer patients. We identified an upregulation of SPHK1, COL4A3, BAI1 and ITGB3, and downregulation of EREG, SERPINF1, CXCL9, CXCL10, IL1B, CCL2, CXCL1, HIF1A and NOTCH4. Postoperative kinetics of angiogenesis related genes in breast cancer Analysis of changes in gene expression between post operative days 3 and 7 revealed overexpression of several circulating transcripts which were previously under expressed.
Inhibitors,Modulators,Libraries Moreover, COL4A3, which inhibitor Oligomycin A was initially upregulated, showed significant downregulation bet ween days 3 and 7. CXCL9, FGF1 and TIMP3 seem to show an opposite trend. Discussion We investigated the impact of mastectomy for breast cancer on circulating molecules of angiogenesis. For this purpose, serial blood measurements were obtained from 10 cancer patients who underwent mastectomy before and up to postsugery day 7, and 6 patients with fibro adenoma who served as a control group. VEGFA is a key player in the process of angiogenesis.