The endospores were obtained from seven days-old
BG and purified of cell debris and the medium. Purity of the endospores was >98 %, verified by microscopic examination. Finally, BG endospores were suspended in GW786034 purchase ethanol, or in purified sterile water, and used for bio-aerosol generation. The densities of the endospore suspensions used for bio-aerosol generation inside the aerosol chamber were in the range of 106–109 colony forming units (CFU) per ml. The transmittance of BG spores described above was measured at Military University of Technology and was used in the interpretation of the measured infrared spectra. The FTIR Spectrometer Constructed for Remote Detection of Bio-aerosols in the Laboratory and in the Field The newly built FTIR spectrometer is a prototype of a SHP099 cost portable field instrument intended to monitor the atmosphere. The instrument works in two spectral channels, namely: 3–5 μm and 8–12 μm atmospheric windows, with spectral resolution of about 1 cm−1. The spectral resolution and other measurement parameters
were chosen to take into account dynamic behaviours of biological agents. Adequate high speed of both, optical path changes and data collection, is necessary. The system enables us to measure between 4 and 8 interferograms per second. Reduction Ro-3306 research buy of the spectral resolution allows optimizing the speed of the measurements. The spectrometer can work as an autonomous system collecting data in its mass memory. Moreover, the measurements can be controlled by software from a portable computer. The spectrometer described above is shown in Fig. 1. The instrument is composed of the measurement head, a control and data acquisition unit, and a telescope allowing to select the target volume. At the front of the measurement head two entrance windows for both channels can be observed. All component parts can be mounted on the tripod. Field of view of both channels is around 1.2 deg. The diameter
of the beam is 25 mm. Fig. 1 FTIR Spectrometer for the detection and identification of bio-aerosols in the atmosphere (Technical University Warsaw, Poland; Space Research Centre, Warsaw, Poland) Passive Remote Detection of Biological Aerosols in the Laboratory The measurements of the radiance from BG were done in the laboratory chamber Flavopiridol (Alvocidib) using our newly constructed FTIR spectrometer. In Fig. 2 the spectrometer with nitrogen cooled detector intended for laboratory measurements is shown. The scheme of our laboratory spectrometric measurements is described in Fig. 3. Biological aerosols were injected into the laboratory cubic chamber in sensor’s field of view. The background spectra were obtained before and after the main measurements. The background in this case was a black body with various temperatures. Fig. 2 Laboratory FTIR spectrometer with nitrogen-cooled detector Fig.