The fraction

powder was also dissolved in methanol, and ginsenoside Rg3 was analyzed by HPLC. HPLC was carried out on an LC system equipped with an autosampler and a binary gradient pump (Capillary HP 1100; Agilent Technologies, Santa Clara, CA, USA). A reversed-phase column (Venusil XBP C18, 250 mm × 4.6 mm, internal diameter 5 μm; Agela Technology, Newark, DE, USA) was used for quantitative determination of ginsenoside Rg3 (20 mg/g). The mobile phase consisted of acetonitrile (A) and water (B) with a flow rate at 1.6 mL/min, and the column was kept constant at 30°C. The detection wavelength was set at 203 nm. We measured the effects of ginseol k-g3 on general locomotor activity. Thirty minutes after drug or saline (control group) administration, separate groups of mice were placed individually in the center of an activity box (measuring 47 cm × 47 cm), bordered by 42-cm high side walls. Spontaneous SP600125 clinical trial activity was measured selleck chemical for 10 min using automated systems (Ethovision System; Noldus Information Technology, Wageningen, Netherlands). The following indices of locomotor activity were recorded by the computer program: moved distance, movement duration, and frequency of rearing. In separate groups of mice, the effects of the repeated (6 d) administration

of ginseol k-g3 on locomotion were also investigated. Locomotor activity tests were conducted during the 1st, 3rd and the final day of drug treatment. Y-maze tests were conducted as described previously [29]. One hour before the tests, mice were administered with the test compounds or with saline or donepezil (positive control). After 30 min, scopolamine [1 mg/kg, intraperitoneally (i.p.)] was injected to induce memory impairment. The effects of the drugs on spontaneous alternation behavior of mice were measured for 8 min. An arm entry was defined as the entry of all four paws and the tail into one arm. The sequence of arm entries was recorded using automated systems (Ethovision System). The alternation Tacrolimus (FK506) behavior (actual alternations) was defined

as the consecutive entry into three arms, that is, the combination of three different arms, with stepwise combinations in the sequence. The maximum number of alternations was considered as the total number of arms entered minus 2, and the percentage of alternation behavior was calculated as (actual alternations/maximum alternations) × 100. The number of arm entries was used as an indicator of locomotor activity. The passive avoidance task was conducted in identical illuminated and nonilluminated boxes (Gemini Avoidance System, San Diego Instruments, San Diego, CA, USA), as described previously [29] and [30]. Mice underwent an acquisition trial and a retention trial that followed 24 h later. One hour before the acquisition trial, mice were given the test drugs, saline (control group) or donezepil.