We compared two different methods, fluorescence in situ hybridization
staining and SYTO11 staining, NVP-BSK805 mouse to describe these new Wolbachia infections in C7-10.
Methods and Results:
Both staining methods were as efficient to stain Wolbachia. A formula was developed to quantify Wolbachia infection. The infection levels in C7-10B and C7-10R differed. The live stain SYTO11 was found to be useful to visualize Wolbachia in replicating host cells. Its potential cytotoxic effect at high concentration was investigated.
Conclusions:
C7-10 supported two Wolbachia infections, constituting new tools to study Wolbachia-host interactions. The different infection levels suggest that wRi and wAlbB have different requirements for their survival in C7-10 host cell line. Observation of SYTO11-stained live cells gave new insights on Wolbachia segregation pattern during host cell mitosis.
Significance and Impact of the Study:
Wolbachia-induced phenotypes in their arthropod and worm hosts could potentially be used to control pest populations. However, the mechanisms underlying these phenotypes are difficult to study because of Wolbachia’s intracellular lifestyle. The Wolbachia infections
in C7-10 described here could be used as in vitro models to investigate Wolbachia biology.”
“Strategic recollection refers to control processes that allow the retrieval of information that is relevant OTX015 solubility dmso for a specific situation. These processes can be studied in memory exclusion tasks, which require the retrieval of particular
kinds of episodic information. In the current study, we investigated strategic recollection in reality monitoring by event-related potentials (ERPs). Participants studied object words, followed by a picture of the denoted object (perceive Selleckchem AR-13324 condition) or followed by the instruction to imagine such a picture (imagine condition). At test, subjects had to identify words of one study condition and to reject words of the second study condition together with newly presented items. Data analysis showed that object names were better identified when items of the perceive condition were targeted. In this test condition, a left parietal old/new effect (the ERP correlate of recollection) was observed only in response to targets. In contrast, both targets and nontargets elicited this old/new effect when items of the imagine condition were targeted. The magnitude of the left parietal old/new effect to nontargets in this condition (but no other left parietal old/new effect) correlated positively with the discrimination indices of both test conditions. In addition, ERPs to targets and nontargets differed at right frontal electrode sites at longer latencies (1500-1800 ms), with more positive ERPs for targets. Findings indicate that subjects retrieved nontarget information in the more difficult task condition, while they relied on target information alone in the less difficult task.