All R remained anti-HBs+ during follow-up. 3 years post-stopping: 17/18 NR were HBeAg+ (13 with normal ALT vs. 4 with elevated ALT), only 1 patient was HBeAg-. 5 years post-stopping: 15/16 NR were HBeAg+ (7 had normal ALT vs. 8 with ALT elevation) and only 1 patient was HBeAg-. 10 years post-stopping: 7/13 NR were HBeAg+ (4 normal ALT vs. 3 had ALT elevation) and 6 achieved HBeAg seroconversion. 13 years post-stopping: 7/13 NR patients were HBeAg+ (only

1 had normal ALT) vs. 6 HBeAg- with HBeAg<1000IU/ ml and normal ALT. 5 HBeAg+ NR received/ing therapy. Methods: Total RNA was extracted from pre-treatment biopsies in patients and 5 healthy controls. HPRT1 and 7 interferon-inducible genes selleck chemicals llc (ISG15, USP18, MxA, OAS2, OAS3, viperin and CXCL10) mRNA expression was measured by quantitative realtime RT-PCR. HBV genotypes and pre-core region mutations were tested by direct sequencing. The results were compared according to genotypes, presence/absence pre-core mutations and outcome 10 years post-stopping therapy (responders vs. HBeAg+ vs. HBeAg-). Results: R had higher viperin mRNA expression and lower CXCL10 expression than NR (viperin: 16.8 vs.0.4, p<0.05; CHIR-99021 concentration CXCL10: 0.62 vs. 1.4,p<0.05). ISG expression was similar across HBV genotypes and irrespective of presence/absence of pre-core mutations.

HBeAg+ NR had higher ISG15 and CXCL10 mRNA expression than HBeAg- (ISG15: 1.96 vs. 0.41, p<0.05; CXCL10: 3.18 vs. 1.2, p<0.05), but lower viperin mRNA expression (0.52 vs. 2.63, p<0.05). Conclusions: High viperin and low CXCL10 mRNA expression in pre-treatment liver biopsy were predicting therapy response and 10 years follow-up outcomes post-IFN based therapy in immunotolerant CHB patients. Disclosures: Ivana Carey - Grant/Research

Support: Gilead, BMS, Roche; Speaking and Teaching: BMS Kosh Agarwal – Consulting: Boehringer-Ingelheim The following people have nothing to disclose: Kate Childs, Sanjay Bansal, Sarah Tizzard, Matthew J. Bruce, Mary Horner, Diego Vergani, Giorgina Mieli-Vergani “
“Oxidative stress plays a pivotal role in the transition from simple steatosis to non-alcoholic steatohepatitis Dichloromethane dehalogenase (NASH). Probucol is a lipid-lowering agent with strong antioxidant properties, and is reported to be effective for the treatment of NASH in several studies. The aim of the present study was to evaluate the efficacy of probucol for the treatment of NASH with dyslipidemia. Twenty-six patients with biopsy-proven NASH accompanied by dyslipidemia were treated with 500 mg of probucol daily for 48 weeks. Body mass index, visceral fat area, liver function tests, serum lipids, fibrosis markers, ferritin, adiponectin, leptin, urinary 8-hydroxy-2′-deoxyguanosine (U-8OHdG) and elasticity were measured periodically during the study. Follow-up liver biopsy was performed in 18 patients. Serum levels of aminotransferases, total cholesterol and U-8OHdG significantly decreased (P < 0.01).