melitensis RNA extracted at late-log phase are on the abscissa. Stat refers to stationary phase, log refers to late-log phase, and gDNA refers to genomic DNA. The R-squared value (0.8841) is displayed in the upper right-hand quadrant of the graph. (DOC 30 KB) Additional file 2: Table A.1. Genes significantly altered in B. melitensis grown in F12K tissue culture medium to late-log phase, compared to stationary phase under the same conditions. (DOC 800 KB) Additional file 3: Hierarchical

cluster of genes from B. melitensis grown to stationary and late-log phases. Hierarchical clustering was performed on normalized Cy3 (transcript) signal intensity values from 8 arrays using Spotfire DecisionSite 8.2 software.

Columns represent samples, and rows represent individual probes/genes. Higher signal ON-01910 in vitro values are shown in red, and lower signal values are shown in green. Note that BIIB057 concentration all four stationary phase samples clustered together and apart from all four log phase cultures (tick line indicates individual growth phase replicate). Numbers in the top left of the figure indicate the number of cluster levels. The number below (-0.913) represents the calculated similarity measure between the two subnodes in each node. (DOC 109 KB) Additional file 4: RT-PCR primers. The table describes the primers used for testing B. melitensis gene expression by Real time – PCR. (DOC 48 KB) References 1. Corbel MJ: Brucellosis: an overview. Emerg Infect Dis 1997,3(2):213–221.CrossRefPubMed 2. Godfroid J, Cloeckaert A, Liautard JP, Kohler S, Fretin D, Walravens K, Garin-Bastuji B, Letesson JJ: From the discovery of the Malta fever’s agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Vet Res 2005, 36:313–326.CrossRefPubMed 3. Moreno E, Stackebrandt E, Dorsch M, Wolters J, Busch M, Mayer H:BMS202 cell line Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria.

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