Peridium (12–)13–18(–20) μm (n = 20) thick at the base, (5–)6–12(–16) μm (n = 20) at the sides; orange- or reddish brown. Cortical tissue (6–)8–16(–22) μm (n = 20) thick, consisting of thick-walled, compressed angular cells 3–10 μm (n = 30) diam of indistinct outline, superposed by a

thin compact, amorphous orange or Peptide 17 supplier reddish layer. Subcortical tissue a t. angularis of subglobose or angular cells (3–)5–11(–13) × (2.5–)4.5–8.5(–10.0) μm (n = 30), hyaline, but orange to reddish just below the surface layer; AZD6244 manufacturer entire tissue above the perithecia (30–)41–67(–77) μm (n = 20) thick. Subperithecial tissue of hyphae with strongly constricted septa and hyaline, refractive, elongate to subglobose cells (7–)12–38(–57) × (6–)8–18(–24) μm (n = 30) with walls ca 1–2 μm thick. Stroma base a hyaline, loose t. intricata of hyphae (2.0–)2.5–5.2(–7.5) μm (n = 30) wide. Asci (60–)68–84(–94) × (3.3–)4.0–4.5(–5.5) μm (n = 60), stipe (4–)7–13(–17) μm (n = 30) long. Ascospores hyaline, JNJ-64619178 in vivo finely spinulose, cells dimorphic; distal cell 3.0–3.8(–4.5) × (2.5–)2.7–3.2(–3.5) μm, l/w (1.0–)1.1–1.3(–1.7) (n = 60), subglobose, broadly ellipsoidal or wedge-shaped; proximal cell (3.3–)3.8–4.7(–5.5) × (2.0–)2.2–2.7(–3.2) μm, l/w (1.3–)1.5–2.0(–2.7) (n = 60), oblong to nearly ellipsoidal, often slightly attenuated toward the base. Cultures and anamorph: optimal growth at 30°C on all

media, also growing at 35°C. On CMD after 72 h 11–12 mm at 15°C, 35–36 mm at 25°C, 47–49 mm at 30°C, 17–19 mm at 35°C; mycelium covering the plate

after 5–6 days at 25°C. Colony hyaline, thin, circular, not zonate, scarcely visible, with little mycelium on the agar surface; hyphae loosely arranged, with conspicuous difference in thickness between primary and secondary hyphae. Distal margin appearing slightly hairy to floccose due to long branched aerial hyphae. Autolytic activity low, coilings conspicuous. A coconut-like odour developing and a yellow pigment diffusing through the agar after 4 days. After 2 weeks the yellow pigment sometimes occurring as long needle-shaped crystals on the agar surface, particularly at higher temperatures. Chlamydospores noted after 6–8 days, Bumetanide scant; see SNA for measurements. Conidiation starting after 2–3 days, effuse; solitary phialides in rows arising from surface hyphae or fascicles of 3–5(–6) phialides from short, erect, scarcely branched conidiophores; within 4–9 days visible as inconspicuous and ill-defined powdery, white to pale yellow granules mainly in the distal third of the plate. Granules 0.1–0.5(–1.0) mm diam, made up of single or few coalescing conidiophores, bearing conidia in heads of up to 60 μm diam and later sometimes in chains. At the same time conidiation also occurring submerged in the agar. Conidiophores to 200 μm long, simple or with up to 5(–7) primary branches, mostly regularly tree-like, i.e.