Similarly, stimulation by IGF-1 increased the proliferation of hepatic progenitor cells with increased expression of Pin1 and other proteins that regulate cell cycle. The results also showed that Everolimus clinical trial Pin1 over expression increased oval cell proliferation, which was further confirmed by increased cell number in G2/M stage of cell cycle in

FACS analysis. Further, Pin1 knockdown by siRNA rendered proliferation of oval cells as confirmed by WST-1 and BrdU incorporation assay. Conclusion: In conclusion, Pin1 protects hepatocyte apoptosis in acute liver injury and help oval cell mediated liver regeneration in an environment that is inhibitory to hepatocyte proliferation in the chronic liver injury. Key Word(s): 1. Pin1; 2. Hepatocyte; 3. 0.1% DDC; 4. liver disease; Presenting GSK1120212 Author: PRABODH RISAL Additional Authors: YEONJUN JEONG Corresponding Author: PRABODH RISAL Objective: Peptidyl-prolyl isomerase, Pin1, a member of parvulin family of PPIase enzyme plays a crucial role in the regulation of post phosphorylation reaction, which governs important role in the cell signalling mechanism. Studies have shown the role of Pin1 in normal as well as in pathological conditions. Here we examined the role of Pin1 in acute and chronic liver injuries. Methods: A single dose of carbon tetrachloride (CCl4) was injected to induce acute liver injury and

apoptosis of hepatocytes in mice. Similarly, 0.1%DDC diet was fed for three weeks to induce chronic liver injury and induction of hepatic progenitor cell in mice. Results: Hepaotycte apoptosis was increased when Pin1 was inhibited by Juglone. Further, over-expression of Pin1 reduced hepatocyte apoptosis both invitro and invivo. Pin1 increased in the liver after three weeks of DDC diet along with the

expansion of hepatic progenitor cell, which was confirmed by the expression of CD44 and A6. Cultured hepatic progenitor cell expressed high level of Pin1 along with other markers like EP-CAM, CK-19 and AFP. Pin1 in the hepatic progenitor cell were more resistant to TGF-β induced degradation compared to hepatocytes. Similarly, stimulation by IGF-1 increased the proliferation of hepatic progenitor click here cells with increased expression of Pin1 and other proteins that regulate cell cycle. The results also showed that Pin1 over expression increased oval cell proliferation, which was further confirmed by increased cell number in G2/M stage of cell cycle in FACS analysis. Further, Pin1 knockdown by siRNA rendered proliferation of oval cells as confirmed by WST-1 and BrdU incorporation assay. Conclusion: In conclusion, Pin1 protects hepatocyte apoptosis in acute liver injury and help oval cell mediated liver regeneration in an environment that is inhibitory to hepatocyte proliferation in the chronic liver injury. Key Word(s): 1. Liver; 2. DDC; 3. Oval cell; 4.