The involvement of both Src and ADAMs

has been reported in normal gastrointestinal epithelial and colon cancer cell lines [60]. Several signalling pathways seem to be important in hepatocarcinomas [19], and there is evidence www.selleckchem.com/products/acy-738.html that both EGFR-mediated mechanisms and the COX/prostaglandin system may be involved in the pathobiology of these tumours [17, 18, 20, 35, 36]. The results of the present study suggest a functional interaction between the EGFR and the prostaglandins. It has been proposed that transactivation can explain the mitogenic effect of GPCR ligands in some cell systems [61] and that it represents a means of diversifying signalling in the cells, by linking the input from a large number of ligands stimulating GPCRs to the pleiotypic and potentially tumorigenic effects of the EGFR [62]. However, there seems to be great variation between cell types with respect to the different pathways involved in the

signalling. We have recently shown that while neurotensin, a GPCR agonist, MK-8931 order activates ERK and Akt in an EGFR-independent way in pancreatic cancer Panc-1 cells, as also found by others [63], and activates ERK and Akt via EGFR transactivation in the colon cancer cell line HT 29, neurotensin uses both EGFR-dependent and -independent pathways in the colon cancer cell line HCT 116 [12]. In the present study we have shown that PGE2 has different ways of stimulating Selleckchem Decitabine the cells, acting by FP-mediated EGFR transactivation in the hepatocarcinoma cells, whereas the effect is mediated mainly via EP3 receptors without any involvement of the EGFR

Apoptosis inhibitor in the hepatocytes [37, 52]. This is further evidence of the diversity of intracellular cross-talk and underscores the importance of investigating such mechanisms in order to better understand the signalling in cancer cells. Conclusion The results indicate that in MH1C1 cells, unlike normal hepatocytes, PGE2 activates the MEK/ERK and PI3K/Akt pathways by transactivation of the EGFR, thus diversifying the GPCR-mediated signal. The data also suggest that the underlying mechanisms in these cells involve FP receptors, PLCβ, Ca2+, Src, and proteinase-mediated release of membrane-associated EGFR ligand(s). Acknowledgements The work was supported by the Norwegian Cancer Society. We thank Eva Østby and Ellen Johanne Johansen for excellent technical assistance. References 1. Daub H, Weiss FU, Wallasch C, Ullrich A: Role of transactivation of the EGF receptor in signalling by G-protein-coupled receptors. Nature 1996,379(6565):557–560.PubMedCrossRef 2. Prenzel N, Zwick E, Daub H, Leserer M, Abraham R, Wallasch C, Ullrich A: EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF. Nature 1999,402(6764):884–888.PubMed 3.