Since Ct values are inversely related to signal selleck products strength, the y-axes are inverted to visually demonstrate a rise in signal over time. coli against ciprofloxacin and tetracycline AST results. The visual results of the macrobroth dilution standard method is shown on the left (A and D), along with the time course results of the ETGA (B and E) and gsPCR (C and F) AST analyses, plotting Ct versus time. Vertical, dashed lines indicate when aliquots were removed for analysis. Since Ct values are inversely related to signal strength, the y-axes are inverted to visually

demonstrate a rise in signal over time. Table 1 Comparison of GS-1101 research buy minimum inhibitory concentration results for MSSA, MRSA and E. aureus ATCC 29213         Bacteria from purified cultures NSC 683864 chemical structure Bacteria harvested from a positive blood culture bottle Drug Phenotype CLSI MIC interpretation Macrobroth MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation         4 hr 6 hr 22 hr 4 hr 6 hr 22 hr 4 hr 6 hr 4 hr 6 hr Oxacillin Susceptible S ≤ 2 R ≥ 4 0.25 S 1 S 0.5 S 0.5 S 0.5 S 1 S 1 S 0.25 S 0.25 S 0.25 S 0.25 S Vancomycin Susceptible S ≤ 2 I = 4-8 R ≥ 16 < 0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.125 S <0.125 S <0.125 S <0.125 S S. aureus NRS241         Bacteria from purified cultures Bacteria

harvested from a positive blood culture bottle Drug Phenotype CLSI MIC interpretation Macrobroth MIC and interpretation ETGA MIC and interpretation Levetiracetam gsPCR MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation         4 hr 6 hr 22 hr 4 hr 6 hr 22 hr 4 hr 6 hr 4 hr 6 hr Oxacillin Resistant S ≤ 2 R ≥ 4 16 R 8 R 16 R > 16 R N/Aa 16 R >16 R 8 R 16 R 8 R 2 Sc (VME) Vancomycin Susceptible S ≤ 2 I = 4-8 R ≥ 16 < 0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25b S <0.25 S <0.25d S <0.25d S E. coli ATCC 25922         Bacteria from purified cultures Bacteria harvested from a positive blood culture bottle Drug Phenotype CLSI MIC interpretation Macrobroth MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation         4 hr 6 hr 22 hr 4 hr 6 hr 22 hr 4 hr 6 hr 4 hr 6 hr Ciprofloxacin Susceptible S ≤ 1 I = 2 R ≥ 4 0.008 S 0.016 S 0.016 S 0.031 S 0.016 S 0.016 S 0.031 S 0.008 S 0.008 S 0.004 S 0.008 S Tetracycline Susceptible S ≤ 4 I = 8 R ≥ 16 1 S 0.5 S 0.5 S 1 S 1 S 1 S 1 S 0.5 S 0.5 S 0.25 S 0.5 S All MIC values are in units of μg/mL.