Visualized proteins were exercised from the gels, and digested wi

Visualized proteins were exercised from the gels, and digested with trypsin according to a method described elsewhere [28]. Mass spectrometric data were analyzed with the MASCOT program (Matrix Science Ltd.). The statistical differences among groups of data were analyzed by one-way analysis of variance (ANOVA),

followed by a Bonferroni posttest, using GraphPad Prism software version 4 (GraphPad Software, Inc.) Acknowledgements We are grateful to Dr. Sottile (University of Rochester Medical Center, NY, USA) for providing the FN-null cells. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Science, and Technology of Japan. References 1. Fukui A, Horiguchi Y: https://www.selleckchem.com/products/cbl0137-cbl-0137.html Bordetella dermonecrotic toxin exerting toxicity through activation of the small GTPase Rho. J Biochem 2004,136(4):415–419.PubMedCrossRef 2. Horiguchi Y, Selleckchem GSK690693 Inoue N, Masuda M, Kashimoto T, Katahira J, Sugimoto N, Matsuda M: Bordetella bronchiseptica dermonecrotizing toxin induces reorganization of actin stress fibers through deamidation of Gln-63 of the GTP-binding protein Rho. Proc Natl Acad Sci USA 1997,94(21):11623–11626.PubMedCrossRef

3. Masuda M, Betancourt L, Matsuzawa T, Kashimoto T, Takao T, Shimonishi Y, Horiguchi Y: Activation of rho through a cross-link with polyamines catalyzed by Bordetella dermonecrotizing toxin. Selleck Tozasertib Embo J 2000,19(4):521–530.PubMedCrossRef 4. Matsuzawa T, Kashimoto

T, Katahira J, Horiguchi Y: Identification of a receptor-binding domain of Bordetella dermonecrotic toxin. Infect Immun 2002,70(7):3427–3432.PubMedCrossRef 5. Kashimoto T, Katahira J, Cornejo WR, Masuda M, Fukuoh A, Matsuzawa T, Ohnishi T, Horiguchi Y: Identification of functional domains of Bordetella dermonecrotizing toxin. Infect Immun 1999,67(8):3727–3732.PubMed 6. Horiguchi Y, Senda T, Sugimoto N, Katahira J, Matsuda M: Bordetella bronchiseptica Demeclocycline dermonecrotizing toxin stimulates assembly of actin stress fibers and focal adhesions by modifying the small GTP-binding protein rho. J Cell Sci 1995,108(Pt 10):3243–3251.PubMed 7. Masuda M, Minami M, Shime H, Matsuzawa T, Horiguchi Y: In vivo modifications of small GTPase Rac and Cdc42 by Bordetella dermonecrotic toxin. Infect Immun 2002,70(2):998–1001.PubMed 8. Brockmeier SL, Register KB, Magyar T, Lax AJ, Pullinger GD, Kunkle RA: Role of the dermonecrotic toxin of Bordetella bronchiseptica in the pathogenesis of respiratory disease in swine. Infect Immun 2002,70(2):481–490.PubMedCrossRef 9. Hanada M, Shimoda K, Tomita S, Nakase Y, Nishiyama Y: Production of lesions similar to naturally occurring swine atrophic rhinitis by cell-free sonicated extract of Bordetella bronchiseptica . Jpn J vet Sci 1979,41(1):1–8. 10.

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