The volume of citrate buffer Al ZD6474 Zactima one weeks sp Ter. Rats in the treatment group and fosinopril rats in both groups were administered with fosinopril or vehicle, respectively by gavage t Possible for 20 weeks. The dose of fosinopril is 1,60 mg / kg. Four rats in model group and a rat in the fosinopril group died of organ failure during the study. The end result of grouping was as follows: control group, model group and fosinopril treatment. At the end of the study, curves Augenheilkunde this blood from the cave each rat, collected, and the left kidney was harvested after in situ perfusion heart. Then the kidneys were removed and weighed, cut into 2 parts for the following histological examination, and metabolomics. W During the experimental period, all animals in K Kept provisional for a temperature of 20 25, humidity 65 69% and were subjected to a period of 12 h light / dark cycle with free access to food and tap water. This study was approved by the Ethics Committee of the China-Japan Friendship Institute of Clinical Medicine approved and implemented in accordance with the Guidelines for the Care and Use of Laboratory Animals. 2.2. Determination of K Body mass, a sample of blood and urine at the end of the experiment the K Body weight of the rats was measured, and glucose values were passed through a Blutzuckerme hunter t Tocco determined. The kidney weight index was calculated by dividing the kidney weight per K Body weight calculated. The rats were housed individually in metabolic K Provisional
for 24 h urine collection, and 24-hour urine protein was located by the Bradford method measured. Acquired curves This blood was centrifuged at 3000 r / min centrifugation for 15 min. The serum was separated and total cholesterol, triglycerides, blood urea, serum creatinine, total protein and albumin were matologieanalysator with a CD-H 1600CS. 2.3. A piece of kidney Renal histology was fixed in neutral buffered formalin, embedded in paraffin, cut into 3 m, and found Rbt with periodic acid-Schiff. Twenty glomeruli were hlt is randomly selected from each rat, And magnitude the mesangial extracellular Ren matrix was identified by PAS-positive material in the mesangium. Glomerulus U Eren and middle thirds of the renal cortex for surface Chen measurements were selected hlt, Using Image Pro Plus 6.0. Care was taken to juxtamedull Ren glomeruli our right to refuse. Then, the percentage of glomerular mesangial matrix occupies the Ren tuft selected hlten Calculated. Tubulo-Sch On the paraffin sections found with PAS 100 mag AREA using a rating system Similar to previously described in detail Rated rbt. 2.4. Metabolomic analysis of adrenal cortex was removed from Benazepril  86541-74-4 another part of the kidney and immediately stored for metabolomics study in isolation. Metabolomic analyzes were identical renal cortex using combined GC-TOF-MS and UPLC-TOF-MS platform using the method of sampling Fresh tissue kidney cortex were subjected weighed and the deactivation of the enzyme frozen in liquid nitrogen storage at until use, when each 800 liters of methanol and 20 L was an internal standard mixture in an added Probenr Hrchen by homogenization with a homogenizer T10 basis twice for 30 s followed in an ice bath. The homogenates were obtained min at 4 overnight and centrifuged at 12,000 rpm for 10 min at 4, and the supernatant was equally divided into two parts wh.