Additional gene prediction analysis and functional add to favorites annotation was performed within the Integrated Microbial Genomes – Expert Review (IMG-ER) platform [40]. Genome properties The draft genome consist of one circular chromosome of 1,880,838 bp length with a 58.8% G+C content (Table 3 and Figure 3). Of the 1,810 genes predicted, 1,751 were protein-coding genes, and 59 RNAs; 12 pseudogenes were also identified. The majority of the protein-coding genes (84.4%) were assigned a putative function while the remaining ones were annotated as hypothetical proteins. The distribution of genes into COGs functional categories is presented in Table 4. Table 3 Genome Statistics Figure 3 Graphical map of the chromosome.
From outside to the center: Genes on forward strand (colored by COG categories), Genes on reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content(black), GC skew (purple/olive). … Table 4 Number of genes associated with the general COG functional categories Insights into the genome sequence Comparative genomics The phylum Synergistetes is one of the more recently proposed phyla in the domain Bacteria, posited only four years ago by Jumas-Bilak et al. [23]. As of today the phylum contains only one order, Synergistales, with one family, Synergistaceae, including 11 genera with 18 species (see Figure 1). The members of the phylum are extremely well characterized on the genomic level, with 12 out of the 18 type strains for the member species having already completed or ongoing genome sequencing projects, one type strain targeted for sequencing (Anaerobacterium thermoterrum) and only four type strains currently not indicated for genome sequencing in the Genomes On Line Database (GOLD) [14].
Here we present a brief comparison of the genome of T. velox with its closest phylogenetic neighbors (according to Figure 1): T. acidamonovorans [17] and A. paucivorans [18]. The genomes of the two recently sequenced Thermanaerovibrio type strains differ only slightly in their size, T. velox having 1.88 Mbp and T. acidaminovorans 1.84 Mbp and their total number of genes, 1,810 and 1,825, respectively. A. paucivorans, on the other hand, has a significantly larger genome with 2,494 genes on 2.63 Mbp. An estimate of the overall similarity between T. velox with both, T. acidaminovorans and A.
paucivorans, was generated with the GGDC-Genome-to-Genome Distance Calculator [41-43]. Entinostat This system calculates the distances by comparing the genomes to obtain HSPs (high-scoring segment pairs) and inferring distances from the set of formulas (1, HSP length / total length; 2, identities / HSP length; 3, identities / total length). For convenience, the GGDC also reports model-based DDH estimates along with their confidence intervals [21,41]. Table 5 shows the results of the pairwise comparison. Table 5 Pairwise comparison of T. velox with T. acidaminovorans and A.